Department of Medical Biochemistry, Medical Faculty, Firat University, Elazig, Turkey.
Department of Medical Biochemistry, Medical Faculty, Adiyaman University, Adiyaman, Turkey.
J Biochem Mol Toxicol. 2022 Feb;36(2):e22961. doi: 10.1002/jbt.22961. Epub 2021 Nov 12.
Colorectal cancer (CRC) incidence is increasing gradually and has been become one of the most common cancers worldwide. Hence, it is important to discover cheap, naturally occurring compounds to be effective in suppressing the devastating effect of colon-related tumors. Rosmarinic acid (RA), one of the compounds of plant origin, possesses attractive features for use as an agent for cancer prevention and treatment. This study investigated the ability of RA to prevent azoxymethane (AOM)-induced rat colon carcinogenesis by evaluating the effect of RA on tumor formation and circulatory oxidant-antioxidant status. Moreover, plasma levels of adiponectin (APN) monocyte chemoattractant protein-1 (MCP-1), interleukin-6 (IL-6) were detected by enzyme linked immunosorbent assay. The animals were divided into three groups: Control, AOM, and AOM + RA. Rats were fed a modified pellet diet (15.8% peanut oil was added to the standard diet) during the experimental period. Colon cancer was formed by applying 15 mg/kg AOM intraperitoneal once a week for 4 weeks in both the CRC group and AOM + RA group. Besides AOM, AOM + RA group received 5 mg/kg body weight RA orally every day during the study. The results showed that adenocarcinoma rates formed 87.5% of the AOM group. With treatment of RA, a reduction in the incidence of adenocarcinoma was observed in the AOM + RA group. The plasma MCP-1, IL-6, and TO levels were significantly higher, APN and TAS levels were significantly lower in the AOM group with respect to controls. In addition, there was a significant increase in TAS levels in the RA treatment group compared to the AOM group. These findings suggested that RA may be beneficial in preventing AOM-induced colon carcinogenesis formation.
结直肠癌（CRC）的发病率逐渐升高，已成为全球最常见的癌症之一。因此，发现廉价的天然化合物，对抑制与结肠相关的肿瘤的破坏性影响具有重要意义。迷迭香酸（RA）是一种植物来源的化合物，具有作为癌症预防和治疗药物的诱人特性。本研究通过评估 RA 对肿瘤形成和循环氧化应激-抗氧化状态的影响，研究了 RA 预防氧化偶氮甲烷（AOM）诱导的大鼠结肠癌发生的能力。此外，通过酶联免疫吸附试验检测血浆脂联素（APN）单核细胞趋化蛋白-1（MCP-1）、白细胞介素-6（IL-6）的水平。动物分为三组：对照组、AOM 组和 AOM+RA 组。在实验期间，所有大鼠均喂食改良的颗粒饲料（标准饲料中添加 15.8%花生油）。CRC 组和 AOM+RA 组每周腹腔注射 15mg/kg AOM 一次，连续 4 周，形成结肠癌。除 AOM 外，AOM+RA 组在研究期间每天口服 5mg/kg 体重 RA。结果显示，AOM 组腺癌发生率为 87.5%。用 RA 治疗后，AOM+RA 组腺癌的发生率降低。与对照组相比，AOM 组的血浆 MCP-1、IL-6 和 TO 水平显著升高，APN 和 TAS 水平显著降低。此外，与 AOM 组相比，RA 治疗组的 TAS 水平显著升高。这些发现表明，RA 可能有益于预防 AOM 诱导的结肠癌形成。
