Department of Mechanical and Materials Engineering, University of Nebraska, Lincoln, NE, 68588, USA.
Department of Biomedical Engineering, University of Connecticut, Farmington, CT, 06030, USA.
Adv Healthc Mater. 2021 May;10(10):e2002152. doi: 10.1002/adhm.202002152. Epub 2021 Feb 28.
Extremity skeletal muscle injuries result in substantial disability. Current treatments fail to recoup muscle function, but properly designed and implemented tissue engineering and regenerative medicine techniques can overcome this challenge. In this study, a nanoengineered, growth factor-eluting bioink that utilizes Laponite nanoclay for the controlled release of vascular endothelial growth factor (VEGF) and a GelMA hydrogel for a supportive and adhesive scaffold that can be crosslinked in vivo is presented. The bioink is delivered with a partially automated handheld printer for the in vivo formation of an adhesive and 3D scaffold. The effect of the controlled delivery of VEGF alone or paired with adhesive, supportive, and fibrilar architecture has not been studied in volumetric muscle loss (VML) injuries. Upon direct in vivo printing, the constructs are adherent to skeletal muscle and sustained release of VEGF. The in vivo printing of muscle ink in a murine model of VML injury promotes functional muscle recovery, reduced fibrosis, and increased anabolic response compared to untreated mice. The in vivo construction of a therapeutic-eluting 3D scaffold paves the way for the immediate treatment of a variety of soft tissue traumas.
四肢骨骼肌损伤会导致严重残疾。目前的治疗方法无法恢复肌肉功能，但经过适当设计和实施的组织工程和再生医学技术可以克服这一挑战。在这项研究中，我们提出了一种纳米工程化的、生长因子洗脱的生物墨水，它利用拉蓬土纳米粘土来控制释放血管内皮生长因子（VEGF），并利用 GelMA 水凝胶作为支持性和粘附性支架，可以在体内交联。该生物墨水与部分自动化手持打印机一起用于体内形成粘附性和 3D 支架。单独或与粘附性、支持性和纤维状结构联合递送 VEGF 的控制释放在容积性肌肉损失（VML）损伤中尚未得到研究。直接体内打印时，这些构建体与骨骼肌黏附，并持续释放 VEGF。与未治疗的小鼠相比，在 VML 损伤的小鼠模型中体内打印肌肉墨水可促进功能性肌肉恢复、减少纤维化和增加合成代谢反应。治疗性洗脱 3D 支架的体内构建为各种软组织创伤的即时治疗铺平了道路。
