Zhang Shun, Wan Junhui, Chen Minjie, Cai Desheng, Xu Junlan, Chen Qi
General Surgery Department, The First Affiliated Hospital of Nanchang University, Nanchang, China.
Obstetrics and Gynaecology Department, The First Affiliated Hospital of Nanchang University, Nanchang, China.
Front Oncol. 2021 Oct 27;11:720447. doi: 10.3389/fonc.2021.720447. eCollection 2021.
Tumor-infiltrating immune cells, associated with tumor progression, are promising prognostic biomarkers. However, the relationship between levels of gene expression and that of immune cell infiltration in cervical cancer prognosis is unknown. In this study, three cervical cancer gene expression microarrays (GSE6791, GSE63678 and GSE55940) were obtained from the GEO database. The IDO1 gene was identified by differentially expressed gene screening. The gene expression profiles of TCGA and GTEx databases along with comprehensive bioinformatics analysis identified that the IDO1 gene was upregulated in cervical cancer with significant difference in expression at different N stages. In addition, it was also upregulated in HPV16 positive sample. The pan-cancer analysis identified that IDO1 was highly expressed in most cancers. TIMER analysis revealed that the expression of IDO1 in CESC shows positive correlation with CD8 T cells, CD4 T cells, neutrophils, dendritic cells. IDO1 expression showed remarkable positive correlation with all immune cell markers except M1 macrophages. CD8 T cell infiltration GSEA results showed that IDO1 was mainly associated with tumor immune-related signaling pathways.
与肿瘤进展相关的肿瘤浸润免疫细胞是很有前景的预后生物标志物。然而，宫颈癌预后中基因表达水平与免疫细胞浸润水平之间的关系尚不清楚。在本研究中，从GEO数据库获得了三个宫颈癌基因表达微阵列（GSE6791、GSE63678和GSE55940）。通过差异表达基因筛选鉴定出IDO1基因。TCGA和GTEx数据库的基因表达谱以及综合生物信息学分析表明，IDO1基因在宫颈癌中上调，在不同N分期的表达存在显著差异。此外，它在HPV16阳性样本中也上调。泛癌分析表明，IDO1在大多数癌症中高表达。TIMER分析显示，IDO1在CESC中的表达与CD8 T细胞、CD4 T细胞、中性粒细胞、树突状细胞呈正相关。IDO1表达与除M1巨噬细胞外的所有免疫细胞标志物均呈显著正相关。CD8 T细胞浸润GSEA结果表明，IDO1主要与肿瘤免疫相关信号通路有关。
