Chan C C, Wetzig R P, Palestine A G, Kuwabara T, Nusenblatt R B
Laboratory of Immunology, National Eye Institute, Bethesda, MD 20892.
Arch Ophthalmol. 1987 Oct;105(10):1398-402. doi: 10.1001/archopht.1987.01060100100036.
An eye from a patient with systemic sarcoidosis and active ocular involvement was studied by the use of monoclonal antibodies and the immunoperoxidase technique to identify the subtypes of infiltrating cells and the presence of lymphokines. Large numbers of lymphoid cells reacting with anti-pan T cell (Leu-4) and anti-T helper/inducer (Leu-3a) subsets were observed around and within sarcoid granulomas. Only rare T suppressor/cytotoxic cells (Leu-2a) were observed. B lymphocytes and plasma cells were present in a few foci. Within the granuloma, T lymphocytes, macrophages, and epithelioid cells showed membrane labeling for interleukin 2 receptors and interferon gamma. We demonstrate the in vivo expression of interleukin 2 receptors and the release of interferon gamma by mononuclear phagocytes and T lymphocytes in ocular sarcoidosis, and we theorize about the activated T helper cell-derived lymphokines in macrophage activation and sarcoid granuloma formation in the eye.
利用单克隆抗体和免疫过氧化物酶技术，对一名患有全身性结节病且眼部有活动性病变的患者的眼睛进行了研究，以确定浸润细胞的亚型和淋巴因子的存在情况。在结节病肉芽肿周围及内部观察到大量与抗全T细胞（Leu-4）和抗T辅助/诱导细胞（Leu-3a）亚群发生反应的淋巴细胞。仅观察到罕见的T抑制/细胞毒性细胞（Leu-2a）。在少数病灶中存在B淋巴细胞和浆细胞。在肉芽肿内，T淋巴细胞、巨噬细胞和上皮样细胞显示出白细胞介素2受体和干扰素γ的膜标记。我们证明了眼部结节病中白细胞介素2受体的体内表达以及单核吞噬细胞和T淋巴细胞释放干扰素γ的情况，并对活化的T辅助细胞衍生的淋巴因子在巨噬细胞活化和眼部结节病肉芽肿形成中的作用进行了理论探讨。
