The College of Medical, Qingdao University, Qingdao, 266021, China.
Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China.
Macromol Biosci. 2020 Oct;20(10):e2000141. doi: 10.1002/mabi.202000141. Epub 2020 Jul 30.
Antibacterial activity and promoting wound healing are two important characteristics of ideal dressings. The previous work has successfully prepared a stem cell seeded polyvinyl alcohol (PVA) hydrogel dressing, which could promote wound healing by active factors secreted from the dressing. However, a lack of antibacterial activity might limit its better application. In this study, a photo-active gelatin (Az-Gel) modified stem cell seeded bilayer PVA hydrogel dressings with silver nanoparticles loaded poly(lactic-co-glycolic acid) (PLGA) electrospinning films (Ag-PLGA) in it is prepared. The physical properties of the dressings show the dressings are mechanically enhanced by the addition of Ag-PLGA film. The addition of Ag-PLGA film does not alter the moisture content of PVA, but extends the moisture of the dressing. Obviously, antibacterial activity is observed and Ag-PLGA/PVA scaffold is biocompatible and low toxic to murine dermal fibroblasts (NIH-3T3). When seeded with Adipose-derived stem cells (ADSCs), bioactive factors secreted by ADSCs could penetrate the dressing and promote cell growth and wound healing. It is speculated that the stem cells seeded bilayer dressing would be of great potential for skin tissue engineering, because of its antibacterial activity and safe application of stem cells.
抗菌活性和促进伤口愈合是理想敷料的两个重要特征。之前的工作已经成功地制备了一种干细胞接种的聚乙烯醇（PVA）水凝胶敷料，它可以通过敷料中分泌的活性因子促进伤口愈合。然而，缺乏抗菌活性可能会限制其更好的应用。在这项研究中，制备了一种光活性明胶（Az-Gel）修饰的干细胞接种双层 PVA 水凝胶敷料，其中含有负载有银纳米粒子的聚（乳酸-共-乙醇酸）（PLGA）电纺膜（Ag-PLGA）。敷料的物理性质表明，Ag-PLGA 膜的加入增强了敷料的机械性能。Ag-PLGA 膜的加入并不改变 PVA 的水分含量，但延长了敷料的水分。显然，观察到了抗菌活性，并且 Ag-PLGA/PVA 支架对鼠真皮成纤维细胞（NIH-3T3）具有生物相容性和低毒性。当接种脂肪来源的干细胞（ADSCs）时，ADSCs 分泌的生物活性因子可以穿透敷料，促进细胞生长和伤口愈合。可以推测，由于其抗菌活性和干细胞的安全应用，干细胞接种双层敷料在皮肤组织工程中具有很大的潜力。
