Gu W Z, Brandwein S R
Abbott Laboratories, Abbott Park, Illinois 60064, USA.
Int J Immunopharmacol. 1998 Aug;20(8):389-400. doi: 10.1016/s0192-0561(98)00035-6.
The effects of purified triptolide, a diterpenoid triepoxide compound derived from the Chinese traditional anti-rheumatic medicinal plant extract, Tripterygium wilfordii Hook f (TWHf), were determined in type II collagen-induced arthritis (CIA) in rats. Lewis rats were immunized with bovine type II collagen and treated with purified triptolide 0.1 mg/kg/day or control (vehicle for triptolide) by daily gavage feedings for 28 days. Triptolide was well-tolerated with no evidence of toxicity. Treatment with triptolide resulted in significant delay in time to onset of arthritis (P = 0.039), as well as significantly decreased arthritis incidence (P = 0.024), clinical arthritis severity score (P < 0.0001), histopathological arthritis severity score (P < 0.0001), and in vivo cell-mediated immunity to collagen (P = 0.0004). Triptolide appeared to be a potent immunomodulatory inhibitor of CIA in rats and this may account for the previously observed anti-rheumatic properties of crude extracts of TWHf, although more extensive studies will be needed to confirm these effects.
从中国传统抗风湿药用植物提取物雷公藤（Tripterygium wilfordii Hook f，TWHf）中提取的二萜类三环氧化合物雷公藤甲素的作用，在大鼠II型胶原诱导的关节炎（CIA）中进行了测定。用牛II型胶原免疫Lewis大鼠，并通过每日灌胃给予0.1mg/kg/天的纯化雷公藤甲素或对照（雷公藤甲素的赋形剂），持续28天。雷公藤甲素耐受性良好，没有毒性证据。雷公藤甲素治疗导致关节炎发病时间显著延迟（P = 0.039），以及关节炎发病率显著降低（P = 0.024）、临床关节炎严重程度评分显著降低（P < 0.0001）、组织病理学关节炎严重程度评分显著降低（P < 0.0001），以及对胶原的体内细胞介导免疫显著降低（P = 0.0004）。雷公藤甲素似乎是大鼠CIA的一种有效的免疫调节抑制剂，这可能解释了先前观察到的TWHf粗提物的抗风湿特性，尽管需要更广泛的研究来证实这些作用。
