Zeng Wenhui, Wu Luyan, Ishigaki Yusuke, Harimoto Takashi, Hu Yuxuan, Sun Yidan, Wang Yuqi, Suzuki Takanori, Chen Hong-Yuan, Ye Deju
State Key Laboratory of Analytical Chemistry for Life Science, Chemistry and Biomedicine Innovation Center (ChemBIC), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, China.
Department of Chemistry, Faculty of Science, Hokkaido University, N10 W8, North-ward, Sapporo, 060-0810, Japan.
Angew Chem Int Ed Engl. 2022 Jan 21;61(4):e202111759. doi: 10.1002/anie.202111759. Epub 2021 Dec 10.
Accurate detection of hepatic hydrogen sulfide (H S) to monitor H S-related enzymes' activity is critical for acute hepatitis diagnosis, but remains a challenge due to the dynamic and transient nature of H S. Here, we report a H S-activatable near-infrared afterglow/MRI bimodal probe F1-GdNP, which shows an "always-on" MRI signal and "off-on" afterglow signal toward H S. F1-GdNP shows fast response, high sensitivity and specificity toward H S, permitting afterglow imaging of H S and evaluation of cystathionine γ-lyase (CSE)'s activity in living mice. We further employ the high spatial-resolution MRI signal of F1-GdNP to track its delivery and accumulation in liver. Importantly, F1-GdNP offers a high signal-to-background ratio (SBR=86.2±12.0) to sensitively report on the increased hepatic H S level in the acute hepatitis mice via afterglow imaging, which correlated well with the upregulated CSE activity in the liver, showcasing the good potential of F1-GdNP for monitoring of acute hepatitis process in vivo.
准确检测肝脏中的硫化氢（H₂S）以监测与H₂S相关的酶的活性对于急性肝炎的诊断至关重要，但由于H₂S的动态和短暂性质，这仍然是一个挑战。在此，我们报告了一种H₂S可激活的近红外余辉/MRI双模态探针F1-GdNP，它对H₂S显示出“始终开启”的MRI信号和“关闭-开启”的余辉信号。F1-GdNP对H₂S表现出快速响应、高灵敏度和特异性，允许对H₂S进行余辉成像并评估活体小鼠中胱硫醚γ-裂解酶（CSE）的活性。我们进一步利用F1-GdNP的高空间分辨率MRI信号来追踪其在肝脏中的递送和积累。重要的是，F1-GdNP提供了高的信背比（SBR = 86.2±12.0），通过余辉成像灵敏地报告急性肝炎小鼠肝脏中H₂S水平的升高，这与肝脏中上调的CSE活性密切相关，展示了F1-GdNP在体内监测急性肝炎过程的良好潜力。
