State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210023, P. R. China.
Chem Asian J. 2020 Nov 2;15(21):3551-3557. doi: 10.1002/asia.202000964. Epub 2020 Oct 6.
Employing a sequentially activated probe design method, an activatable, switchable and dual-mode probe was designed. This nanoprobe, HSDPP, could be effectively activated by H S to form H-type aggregates with green emission; subsequently, the aggregates could bind to mtDNA to form monomers and the emIssion color switched from green to deep-red. We exploited HSDPP to image exogenous and endogenous H S in living cells. Of note, for the first time, this novel nanoprobe with an optimal partition coefficient value (LogP=1.269) was successfully applied for tracking the endogenous H S upregulation stimulated by cystathionase activator S-adenosyl-L-methionine (SAM) in mice brains. Finally, our work provides an invaluable chemical tool for probing endogenous H S upregulation in vitro/vivo and, importantly, affords a prospective design strategy for developing switchable chemosensors to unveil the relationship between biomolecules and DNA in mitochondria in many promising areas.
采用顺序激活探针设计方法，设计了一种可激活、可切换的双模式探针 HSDPP。该纳米探针可被 H 2 S 有效激活，形成具有绿色发射的 H 型聚集物；随后，聚集物可以与 mtDNA 结合形成单体，发射颜色从绿色变为深红色。我们利用 HSDPP 在外源和内源性 H 2 S 在活细胞中的成像。值得注意的是，该新型纳米探针具有最佳分配系数值（LogP=1.269），首次成功应用于跟踪胱硫醚酶激活剂 S-腺苷-L-蛋氨酸（SAM）刺激的内源性 H 2 S 上调在小鼠大脑中。最后，我们的工作为探测体外/体内内源性 H 2 S 上调提供了一个非常有价值的化学工具，并且为开发可切换化学传感器提供了一个有前景的设计策略，以揭示线粒体中生物分子与 DNA 之间的关系，这在许多有前途的领域具有重要意义。
