Wu Chuan, Zhou Yong, Tian Tian, Li Ting-Jing, Chen Bin
Chongqing Key Laboratory of Vector Insects, Institute of Entomology and Molecular Biology, Chongqing Normal University, Chongqing, China.
Insect Sci. 2022 Aug;29(4):1226-1238. doi: 10.1111/1744-7917.12983. Epub 2021 Dec 20.
Despite worldwide distribution and rich diversity, the knowledge of mitochondrial genome (mtgenome) characteristics within the family Tenebrionidae (Coleoptera) is still very limited, and phylogenetics remain unresolved for the family. In this study, the mtgenomes for 12 species are newly sequenced and annotated. Three of the species represent the first report of complete mtgenomes from the subfamily Alleculinae. Comparative analysis of 36 tenebrionid mtgenomes finds that gene composition and order are the same as a theoretical ancestral insect mtgenome, and AT bias, length variation, and codon usage are consistent with other reported beetle mtgenomes. Some intergenic overlap and gap sequences may contain phylogenetically informative information, whereas neither the conserved nor repeat sequences in the control region do. The subfamilies Lagriinae, Pimeliinae, Stenochiinae, and Alleculinae are found to be monophyletic, and the subfamilies Diaperinae and Tenebrioninae polyphyletic in our study. Furthermore, Lagriinae is sister to the rest of the subfamilies. At the tribal level, the tribes Lagriini in the subfamily Lagrrinae; Strongyliini in Stenochiinae; Cteniopodini in Alleculinae; and Triboliini, Opatrini, and Amarygmini in Tenebrioninae are monophyletic; while Diaperini in Diaperinae, and Tenebrionini in Tenebrioninae are polyphyletic.
尽管拟步甲科（鞘翅目）线粒体基因组（mtgenome）具有全球分布且多样性丰富的特点，但其相关知识仍非常有限，该科的系统发育关系也尚未明确。在本研究中，新测序并注释了12个物种的线粒体基因组。其中三个物种代表了扁甲亚科完整线粒体基因组的首次报道。对36个拟步甲线粒体基因组的比较分析发现，基因组成和顺序与理论上的昆虫祖先线粒体基因组相同，AT偏好、长度变异和密码子使用情况与其他已报道的甲虫线粒体基因组一致。一些基因间重叠和间隔序列可能包含系统发育信息，而控制区的保守序列和重复序列均不包含。在本研究中，发现毛薪甲亚科、皮蠹亚科、窄胸甲亚科和扁甲亚科是单系的，而花甲亚科和拟步甲亚科是多系的。此外，毛薪甲亚科是其他亚科的姐妹群。在族级水平上，毛薪甲亚科的毛薪甲族；窄胸甲亚科的强脊甲族；扁甲亚科的栉足甲族；以及拟步甲亚科的拟谷盗族、斑蠹族和阿玛粒甲族是单系的；而花甲亚科的花甲族和拟步甲亚科的拟步甲族是多系的。
