Department of Biotechnology, College of Life Science and Biotechnology, Korea University, Seoul 02841, Republic of Korea; National Academy of Agricultural Science, Rural Development Administration, Wanju-gun 55365, Republic of Korea.
National Academy of Agricultural Science, Rural Development Administration, Wanju-gun 55365, Republic of Korea.
Biomed Pharmacother. 2021 Dec;144:112365. doi: 10.1016/j.biopha.2021.112365. Epub 2021 Oct 28.
In this study, we investigated the effects of radish sprout ethanol extract (RSE) on inflammatory responses in the macrophages and a mouse model of colitis. RSE administration was found to effectively inhibit the phosphorylation of IκB and, in turn, the production of pro-inflammatory enzymes and cytokines in lipopolysaccharide-stimulated macrophages. In dextran sulfate sodium (DSS)-colitis mice, RSE administration prevented body weight and colon length reduction, while decreasing inflammation and mucosal necrosis. The diversity of the fecal microbiota was significantly increased in the group treated with RSE. In addition, RSE administration decreased the relative abundance of the phylum Proteobacteria, which includes many pathogens, and increased the abundance of the genus Akkermansia. Beta diversity analyses showed that RSE administration restored the gut microbiota composition close to that of healthy mice. For the first time, we identified glycosides of sinapic acid as part of hydroxycinnamic acids in RSE with colitis-alleviating effects. Notably, 1,2-O-disinapoyl glucoside substantially decreased nitric oxide generation in LPS-stimulated macrophages.
在这项研究中，我们研究了萝卜芽乙醇提取物（RSE）对巨噬细胞炎症反应和结肠炎小鼠模型的影响。研究发现，RSE 给药可有效抑制 IκB 的磷酸化，进而抑制脂多糖刺激的巨噬细胞中促炎酶和细胞因子的产生。在葡聚糖硫酸钠（DSS）-结肠炎小鼠中，RSE 给药可预防体重和结肠长度的减少，同时减轻炎症和粘膜坏死。用 RSE 处理的粪便微生物组的多样性显著增加。此外，RSE 给药降低了包括许多病原体在内的厚壁菌门的相对丰度，并增加了阿克曼氏菌属的丰度。β多样性分析表明，RSE 给药使肠道微生物组组成恢复到接近健康小鼠的水平。我们首次鉴定出 RSE 中具有缓解结肠炎作用的芥子酸糖苷是羟基肉桂酸的一部分。值得注意的是，1,2-O-二芥子酰基葡萄糖苷可显著减少 LPS 刺激的巨噬细胞中一氧化氮的生成。
