Liu Tan, Yin Yixuan, Yang Yang, Russell Thomas P, Shi Shaowei
Beijing Advanced Innovation Center for Soft Matter Science and Engineering, College of Materials Science and Engineering, Beijing University of Chemical Technology, Beijing, 100029, China.
Department of Polymer Science and Engineering, University of Massachusetts, Amherst, MA, 01003, USA.
Adv Mater. 2022 Feb;34(5):e2105386. doi: 10.1002/adma.202105386. Epub 2021 Dec 22.
Enzyme immobilization in the confines of microfluidic chips, that promote enzyme activity and stability, has become a powerful strategy to enhance biocatalysis and biomass conversion. Here, based on a newly developed all-liquid microfluidic chip, fabricated by the interfacial assembly of nanoparticle surfactants (NPSs) in a biphasic system, a layer-by-layer assembly strategy to generate polysaccharide multilayers on the surface of a microchannel, greatly enhancing the mechanical properties of the microchannel and offering a biocompatible microenvironment for enzyme immobilization, is presented. Using horseradish peroxidase and glucose oxidase as model enzymes, all-liquid microfluidic enzymatic and cascade reactors have been constructed and the crucial role of polysaccharide multilayers on enhancing the enzyme loading and catalytic efficiency is demonstrated.
将酶固定在微流控芯片的受限空间内，可促进酶的活性和稳定性，这已成为增强生物催化和生物质转化的有力策略。在此，基于一种新开发的全液微流控芯片，该芯片由双相系统中纳米颗粒表面活性剂（NPS）的界面组装制成，提出了一种在微通道表面生成多糖多层膜的逐层组装策略，极大地增强了微通道的机械性能，并为酶固定提供了生物相容性微环境。以辣根过氧化物酶和葡萄糖氧化酶为模型酶，构建了全液微流控酶促和级联反应器，并证明了多糖多层膜在提高酶负载量和催化效率方面的关键作用。
