Vinča Institute of Nuclear Sciences - National Institute of the Republic of Serbia, University of Belgrade, Belgrade, Serbia.
Vinča Institute of Nuclear Sciences - National Institute of the Republic of Serbia, University of Belgrade, Belgrade, Serbia; Texas A&M University at Qatar, P.O. Box 23874, Doha, Qatar.
Int J Biol Macromol. 2022 Jan 1;194:461-469. doi: 10.1016/j.ijbiomac.2021.11.089. Epub 2021 Nov 18.
Aqueous colloids, consisting of 15-30 nm-sized silver nanoparticles (Ag NPs), were prepared using the reducing and stabilizing abilities of glucose, sucrose, and dextran. The long-term stability of coated Ag NPs increases from glucose over sucrose to dextran, i.e., with the increase of the molecular weight of carbohydrate molecules. The density functional theory (DFT) calculations of the partial atomic (Mulliken) charges and adsorption energies are applied to explain the enhanced stability of coated Ag NPs. All coated Ag NPs have a significantly broader concentration range of nontoxic behavior toward pre-osteoblast cells than bare Ag NPs prepared using sodium borohydride. The carbohydrate-coated Ag NPs display the same level of toxic ability against Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria as bare Ag NPs. The differences in toxicity mechanism of the coated and bare Ag NPs are a consequence of the absence and presence of co-occurring Ag ions in examined dispersion, respectively.
采用葡萄糖、蔗糖和葡聚糖的还原和稳定能力制备了由 15-30nm 尺寸的银纳米粒子（Ag NPs）组成的水性胶体。涂覆的 Ag NPs 的长期稳定性从葡萄糖增加到蔗糖，再增加到葡聚糖，即随着碳水化合物分子的分子量增加而增加。应用密度泛函理论（DFT）计算部分原子（Mulliken）电荷和吸附能来解释涂覆的 Ag NPs 的增强稳定性。所有涂覆的 Ag NPs 对前成骨细胞的非毒性行为的浓度范围明显宽于使用硼氢化钠制备的裸 Ag NPs。与裸 Ag NPs 相比，涂覆有碳水化合物的 Ag NPs 对革兰氏阴性（大肠杆菌）和革兰氏阳性（金黄色葡萄球菌）细菌的毒性能力相同。涂覆的和裸的 Ag NPs 的毒性机制的差异是由于在检查的分散体中分别存在和不存在共存的 Ag 离子所致。
