Jiang Zhu-Ming, Zhang Bing-Huo, Sun Hong-Min, Zhang Tao, Yu Li-Yan, Zhang Yu-Qin
Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.
College of Life Science, Jiujiang University, Jiujiang, China.
Front Microbiol. 2021 Nov 5;12:742798. doi: 10.3389/fmicb.2021.742798. eCollection 2021.
Three Gram-stain-positive, aerobic, motile actinobacterial strains designated as CPCC 205119, CPCC 205215, and CPCC 205251 were isolated from different biological soil crust samples collected from Tengger Desert, China. The 16S rRNA gene sequence comparison of these three strains showed they had almost identical 16S rRNA genes, which were closely related to members of the family , with the highest similarities of 96.3-97.3% to the species of . In the phylogenetic tree based on 16S rRNA gene sequences, these isolates clustered into a subclade next to the branch containing the species of and , within the lineage of the genus . The comparative genomic characteristics (values of ANI, dDDH, AAI, and POCP) and the phenotypic properties (morphological, physiological, and chemotaxonomic characteristics) of these isolates readily supported to affiliate them to the genus as a single separate species. For which, we proposed that the isolates CPCC 205119, CPCC 205215, and CPCC 205251 represent a novel species of the genus as sp. nov. CPCC 205119 (=I12A-02624=NBRC 113528=KCTC 49201) is the type strain. The genome of strain CPCC 205119 consisted of one chromosome (4,843,235bp) containing 4,424 coding genes, 48 tRNA genes, five rRNA genes, three other ncRNA genes, and 101 pseudogenes, with G+C content of 74.7%. The whole-genome sequences analysis indicated that this species contained alkaline phosphatase genes (/), phosphate transport-related genes (, , , , , , , , , , , , , and ), trehalose-phosphate synthase gene (), trehalose 6-phosphate phosphatase gene () and other encoding genes for the properties that help the microorganisms to adapt to harsh environmental conditions prevalent in deserts. Strains of this species could solubilize tricalcium phosphate [Ca(PO)] and phytin, assimilate pyrophosphate, thiophosphate, dithiophosphate, phosphoenol pyruvate, 2-deoxy-d-glucose-6-phosphate, and cysteamine-S-phosphate.
从中国腾格里沙漠采集的不同生物土壤结皮样本中分离出三株革兰氏阳性、需氧、运动的放线菌菌株，分别命名为CPCC 205119、CPCC 205215和CPCC 205251。这三株菌株的16S rRNA基因序列比较显示，它们具有几乎相同的16S rRNA基因，与[科名]的成员密切相关，与[属名]物种的相似度最高达96.3 - 97.3%。在基于16S rRNA基因序列的系统发育树中，这些分离株聚集成一个亚分支，紧邻包含[属名]物种的分支，位于[属名]的谱系内。这些分离株的比较基因组特征（ANI、dDDH、AAI和POCP值）和表型特性（形态、生理和化学分类特征）很容易支持将它们归为[属名]的一个单独新物种。为此，我们提议分离株CPCC 205119、CPCC 205215和CPCC 205251代表[属名]的一个新物种，命名为[新物种名] sp. nov.。CPCC 205119（=I12A - 02624=NBRC 113528=KCTC 49201）是模式菌株。菌株CPCC 205119的基因组由一条染色体（4,843,235bp）组成，包含4,424个编码基因、48个tRNA基因、5个rRNA基因、3个其他非编码RNA基因和101个假基因，G + C含量为74.7%。全基因组序列分析表明，该物种含有碱性磷酸酶基因（/）、磷酸盐转运相关基因（[多个基因名称列举]）、海藻糖磷酸合酶基因（）、海藻糖6 - 磷酸磷酸酶基因（）以及其他有助于微生物适应沙漠中普遍存在的恶劣环境条件的特性的编码基因。该物种的菌株能够溶解磷酸三钙[Ca(PO)]和肌醇六磷酸，同化焦磷酸、硫代磷酸、二硫代磷酸、磷酸烯醇丙酮酸、2 - 脱氧 - d - 葡萄糖 - 6 - 磷酸和半胱胺 - S - 磷酸。
