School of Pharmacy, Qilu Medical University, Zibo 255300, PR China.
College of Pharmaceutical Science & Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Zhejiang University of Technology, Hangzhou 310014, PR China.
Mar Genomics. 2021 Jun;57:100830. doi: 10.1016/j.margen.2020.100830. Epub 2020 Nov 5.
Micromonospora craniellae LHW63014 is a novel marine Micromonospora, isolated from a Craniella species sponge collected in the South China Sea. In this study, we report the complete genome sequence of M. craniellae LHW63014, which is comprised of a circular chromosome of 6,839,926 bp with the G + C content of 70.9 mol%. The complete genome contained 6572 protein-coding genes, 48 tRNA genes, and 9 rRNA genes. Genomic annotations revealed that 79.09% of the protein-coding genes were assigned to the COG database, among which, the abundant genes were predicted to be involved in transcription, replication, recombination and repair, and amino acid transport and metabolism. Secondary metabolites prediction using antiSMASH revealed that 22 biosynthetic gene clusters (BGC) of secondary metabolites were located in the genome of M. craniellae LHW63014, 19 of which showed low similarity (<50%) to known BGCs and 5 of which showed the closest homology with BGCs encoding metal ion-chelating agents, indicating the immense potential of M. craniellae LHW63014 to produce a wide variety of novel antibiotics, especially for metal ion-chelating agents.
从南海采集的 Craniella 种海绵中分离得到的新型海洋 Micromonospora 菌株 Micromonospora craniellae LHW63014。在本研究中，我们报告了 M. craniellae LHW63014 的完整基因组序列，该序列由一个 6839926bp 的圆形染色体组成，G+C 含量为 70.9mol%。完整基因组包含 6572 个蛋白编码基因、48 个 tRNA 基因和 9 个 rRNA 基因。基因组注释表明，79.09%的蛋白编码基因被分配到 COG 数据库，其中丰富的基因被预测参与转录、复制、重组和修复以及氨基酸的运输和代谢。使用 antiSMASH 进行次生代谢产物预测表明，M. craniellae LHW63014 的基因组中存在 22 个次生代谢产物生物合成基因簇（BGC），其中 19 个与已知 BGC 的相似度<50%，5 个与编码金属离子螯合剂的 BGC 具有最接近的同源性，这表明 M. craniellae LHW63014 具有产生各种新型抗生素的巨大潜力，特别是金属离子螯合剂。
