Deng Yang, Jiang Zhu-Ming, Han Xue-Fei, Su Jing, Yu Li-Yan, Liu Wei-Hong, Zhang Yu-Qin
Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
State Key Laboratory of Dao-di Herb, Beijing, China.
Front Microbiol. 2023 Feb 28;14:1119226. doi: 10.3389/fmicb.2023.1119226. eCollection 2023.
Five Gram-stain-positive, aerobic, non-motile actinobacterial strains designated as CPCC 205763, CPCC 203386, CPCC 205716, CPCC 203406, and CPCC 203407 were obtained from different ecosystems associated with four kinds of Chinese traditional medicinal plants. The 16S rRNA gene sequences of these five strains showed closely related to members of the genus of the family , with the highest similarities of 97.4-99.7% to the four validly named species of . In the phylogenetic trees based on 16S rRNA gene sequences and the core genome, these isolates clustered into the clade of the genus within the lineage of the family The overall genome relatedness indexes (values of ANI and dDDH) and the phenotypic properties (morphological, physiological and chemotaxonomic characteristics) of these isolates, readily supported to affiliate them to the genus , representing four novel species, with the isolates CPCC 203406 and CPCC 203407 being classified in the same species. For which the names sp. nov. (type strain CPCC 205763 = I19A-01430 = CGMCC 1.60067), sp. nov. (type strain CPCC 203386 = I10A-01569 = DSM 24546 = KCTC 19839), sp. nov. (type strain CPCC 205716 = I21A-01427 = CGMCC 1.60064), and sp. nov. (type strain CPCC 203406 = I10A-02268 = DSM 24549 = KCTC 19840) were proposed, respectively. In the genomes of these five strains, the putative encoding genes for amidase, endoglucanase, phosphatase, and superoxidative dismutase were retrieved, which were classified as biosynthetic genes/gene-clusters regarding plant growth-promotion (PGP) functions. The positive results from IAA-producing, cellulose-degrading and anti-oxidation experiments further approved their potential PGP bio-functions. Pangenome analysis of the genus supported the polyphasic taxonomy results and confirmed their bio-function potential.
从与四种中国传统药用植物相关的不同生态系统中获得了五株革兰氏染色阳性、需氧、不运动的放线菌菌株，分别命名为CPCC 205763、CPCC 203386、CPCC 205716、CPCC 203406和CPCC 203407。这五株菌株的16S rRNA基因序列与[科名]属的成员密切相关，与[属名]四个有效命名物种的最高相似度为97.4 - 99.7%。在基于16S rRNA基因序列和核心基因组的系统发育树中，这些分离株聚集在[科名]科谱系内的[属名]属分支中。这些分离株的全基因组相关性指数（ANI和dDDH值）以及表型特征（形态、生理和化学分类特征），很容易支持将它们归入[属名]属，代表四个新物种，其中分离株CPCC 203406和CPCC 203407被归为同一物种。为此，分别提出了[新物种名1] sp. nov.（模式菌株CPCC 205763 = I19A - 01430 = CGMCC 1.60067）、[新物种名2] sp. nov.（模式菌株CPCC 203386 = I10A - 01569 = DSM 24546 = KCTC 19839）、[新物种名3] sp. nov.（模式菌株CPCC 205716 = I21A - 01427 = CGMCC 1.60064）和[新物种名4] sp. nov.（模式菌株CPCC 203406 = I10A - 02268 = DSM 24549 = KCTC 19840）的名称。在这五株菌株的基因组中，检索到了酰胺酶、内切葡聚糖酶、磷酸酶和超氧化物歧化酶的推定编码基因，这些基因被归类为具有促进植物生长（PGP）功能的生物合成基因/基因簇。产IAA、纤维素降解和抗氧化实验的阳性结果进一步证实了它们潜在的PGP生物功能。对[属名]属的泛基因组分析支持了多相分类学结果，并证实了它们的生物功能潜力。
