Shlomchik M J, Aucoin A H, Pisetsky D S, Weigert M G
Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, PA 19111.
Proc Natl Acad Sci U S A. 1987 Dec;84(24):9150-4. doi: 10.1073/pnas.84.24.9150.
Four monoclonal anti-DNA antibodies derived from a single autoimmune MRL/lpr mouse were studied. Three of these antibodies showed similarities in DNA binding; the fourth had a much higher specific activity for single-stranded DNA and, in addition, was unique in binding double-stranded DNA and cardiolipin. Complete nucleotide sequences of heavy- and light-chain variable regions demonstrated that all four antibodies are clonally related. The sequences also showed numerous somatic mutations, the distribution of which suggests that positive selection by antigen operated on these clonally related autoantibodies.
对源自一只自身免疫性MRL/lpr小鼠的四种单克隆抗DNA抗体进行了研究。其中三种抗体在DNA结合方面表现出相似性；第四种对单链DNA具有更高的比活性，此外，在结合双链DNA和心磷脂方面具有独特性。重链和轻链可变区的完整核苷酸序列表明，所有四种抗体都是克隆相关的。序列还显示出许多体细胞突变，其分布表明抗原的阳性选择作用于这些克隆相关的自身抗体。
