Department of Neurology, Ganzhou People's Hospital, Ganzhou 341000, Jiangxi Province, China.
Department of Pharmacy, Ganzhou Fifth People's Hospital, Ganzhou 341000, Jiangxi Province, China.
Biomed Res Int. 2021 Nov 13;2021:8171236. doi: 10.1155/2021/8171236. eCollection 2021.
This study is set out to explore the potential difference of miR in PD through GEO data and provide diagnostic indicators for clinical practice.
In this study, differential miR was screened through the Gene Expression Omnibus (GEO) database, 68 PD patients treated in our hospital from May 2017 to March 2018 were collected as the research group (RG), and 50 normal subjects who underwent physical examination in our hospital during the same period were collected as the control group (CG). Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expression and diagnostic value of miR-374a-5p in serum of patients. The potential target genes of miR-374a-5p were predicted, and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis and Gene Ontology Consortium (GO) were carried out.
GEO2R analysis revealed that 193 miRs are expressed differentially, of which 78 were highly expressed and 115 were poorly expressed. The miR-374a-5p expression in the serum of the RG was reduced markedly and had a diagnostic value. Targetscan and miRDB online websites were used to predict their target genes, with 415 common target genes. miR-374a-5p may participate in 27 functional pathways and 8 signal pathways.
miR-335-5p has low expression in PD and is expected to be a potential diagnostic indicator.
本研究通过 GEO 数据探讨 PD 中 miR 的潜在差异，为临床实践提供诊断指标。
本研究通过基因表达综合数据库（GEO）筛选差异表达的 miR，收集 2017 年 5 月至 2018 年 3 月我院治疗的 68 例 PD 患者作为研究组（RG），同期我院体检的 50 例正常受试者作为对照组（CG）。采用实时定量聚合酶链反应（qRT-PCR）检测患者血清中 miR-374a-5p 的表达及诊断价值。预测 miR-374a-5p 的潜在靶基因，并进行京都基因与基因组百科全书（KEGG）分析和基因本体联合会（GO）分析。
GEO2R 分析显示，有 193 个 miRs 表达差异，其中 78 个高表达，115 个低表达。RG 血清中 miR-374a-5p 表达明显降低，具有诊断价值。靶标扫描和 miRDB 在线网站预测其靶基因，有 415 个共同靶基因。miR-374a-5p 可能参与 27 个功能途径和 8 个信号途径。
miR-335-5p 在 PD 中低表达，有望成为潜在的诊断指标。
