Laboratory of Animal Pharmacology and Toxicology, Brazil University, Descalvado, SP, Brazil.
Department of Pharmacology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP, Brazil.
Methods Mol Biol. 2022;2411:175-194. doi: 10.1007/978-1-0716-1888-2_10.
Advances in vaccine development depend on animal models to test innovative therapies. Recent studies have reported the successful introduction of teleost fish as a new vertebrate model in scientific research, with emphasis on the species Danio rerio (zebrafish). This chapter aims to give an overview of important aspects related to the immune system of fish, as well as the current progress of the successful use of these animals in studies for the development of vaccines, assisting in the determination of efficacy and clinical safety. Among the advantages of using fish for the development of vaccines and immunomodulatory drugs, it is worth highlighting the reproductive capacity of these animals resulting in a high number of individuals belonging to the same spawning, transparent embryos, low cost of breeding and high genetic similarity that favor translational responses to vertebrate organisms like humans.
疫苗开发的进展取决于动物模型来测试创新疗法。最近的研究报告称，成功地将硬骨鱼引入科学研究中作为一种新型脊椎动物模型，重点是鱼类 Danio rerio（斑马鱼）。本章旨在概述与鱼类免疫系统相关的重要方面，以及这些动物在疫苗开发研究中成功应用的最新进展，以协助确定疗效和临床安全性。在使用鱼类开发疫苗和免疫调节剂方面的优势中，值得强调的是这些动物的繁殖能力导致同一产卵群体中有大量个体、透明胚胎、低繁殖成本和高度的遗传相似性，有利于对像人类这样的脊椎动物产生转化反应。
