Department of Basic Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
Molecules. 2023 Feb 10;28(4):1720. doi: 10.3390/molecules28041720.
Lipopolysaccharide (LPS) has been considered the primary agent to establish animal models of inflammation, immunological stress, and organ injury. Previous studies have demonstrated that LPS impaired gastrointestinal development and disrupted intestinal microbial composition and metabolism. Ferulic acid (FA) isolated from multiple plants exhibits multiple biological activities. This study investigated whether FA ameliorated intestinal function and microflora in LPS-challenged Tianfu broilers. The results showed that LPS challenge impaired intestinal function, as evidenced by decreased antioxidant functions ( < 0.05), disrupted morphological structure ( < 0.05), and increased intestinal permeability ( < 0.05); however, these adverse effects were improved by FA supplementation. Additionally, FA supplementation preserved sIgA levels ( < 0.05), increased mRNA expression levels of and ( < 0.05), and enhanced epithelial proliferation ( < 0.05) in the ileal mucosa in LPS-challenged chickens. Moreover, FA supplementation rectified the ileal microflora disturbances in the LPS-challenged broilers. The results demonstrate that dietary FA supplementation decreased LPS-induced intestinal damage by enhancing antioxidant capacity and maintaining intestinal integrity. Furthermore, FA supplementation protects intestinal tight junctions (TJs), elevates secretory immunoglobulin A (sIgA) levels, and modulates ileal microflora composition in LPS-challenged broilers.
脂多糖 (LPS) 被认为是建立炎症、免疫应激和器官损伤动物模型的主要试剂。先前的研究表明，LPS 损害了胃肠道的发育，破坏了肠道微生物的组成和代谢。从多种植物中分离出的阿魏酸 (FA) 具有多种生物学活性。本研究探讨了 FA 是否能改善 LPS 应激天府肉鸡的肠道功能和微生物群。结果表明，LPS 挑战会损害肠道功能，表现为抗氧化功能下降（<0.05）、形态结构破坏（<0.05）和肠道通透性增加（<0.05）；然而，FA 补充剂可以改善这些不利影响。此外，FA 补充剂可以维持 LPS 应激鸡的 sIgA 水平（<0.05），增加 和 （<0.05）的 mRNA 表达水平，并增强回肠黏膜的上皮细胞增殖（<0.05）。此外，FA 补充剂纠正了 LPS 应激肉鸡回肠微生物群的紊乱。结果表明，膳食 FA 补充剂通过增强抗氧化能力和维持肠道完整性来减少 LPS 诱导的肠道损伤。此外，FA 补充剂可以保护肠道紧密连接 (TJ)，提高分泌型免疫球蛋白 A (sIgA) 水平，并调节 LPS 应激肉鸡的回肠微生物群组成。
