Khobjai Warachate, Ninlaor Wanvisa, Watcharasamphankul Worawan, Thongom Thaksaorn, Sukati Suriyan
Department of Preclinic, Faculty of Medicine, Siam University, Phasi Charoen, Bangkok, Thailand.
Department of Thai Traditional Medicine, Thai Traditional Medicine College, Rajamangala University of Technology Thanyaburi, Pathum Thani, Thailand.
J Adv Pharm Technol Res. 2021 Oct-Dec;12(4):420-424. doi: 10.4103/japtr.japtr_95_21. Epub 2021 Oct 20.
Platelets form a plug to prevent blood loss and contribute to wound healing. Kradonbok, Roxb., is a Thai plant with medicinal properties. Conventionally, leaves of are being used to help wound healing in Thailand. The present study was aimed to investigate the effect of on the function of platelet. Four different extracts of leaves of (distilled water, methanol, ethanol, and chloroform extracts) were prepared. The extracts at 5.0 mg/ml per dose were tested for the effect of on platelet adhesion and aggregation properties, by employing a microtiter plate approach. The phytochemical identification was done by using gas chromatography-mass spectrometry (GC-MS). Our data revealed that chloroform extract significantly activated thrombin-induced platelet adhesion (105.27 ± 0.11%, < 0.05). None of the extracts exhibited an improvement in platelet aggregation. Further GC-MS analysis of the chloroform extract revealed five key phytochemical constituents with potential platelet activation properties. In conclusion, our study evaluated platelet activation and potentially wound healing property of . GC-MS analysis identified potential bioactive phytochemical compounds in which warrant further investigation to characterize these compounds.
血小板形成凝块以防止失血并促进伤口愈合。克拉东博克（Kradonbok, Roxb.）是一种具有药用特性的泰国植物。传统上，在泰国，其叶子被用于帮助伤口愈合。本研究旨在调查其对血小板功能的影响。制备了该植物叶子的四种不同提取物（蒸馏水提取物、甲醇提取物、乙醇提取物和氯仿提取物）。通过采用微量滴定板法，测试每剂量5.0毫克/毫升的提取物对血小板黏附和聚集特性的影响。使用气相色谱 - 质谱联用仪（GC - MS）进行植物化学鉴定。我们的数据显示，氯仿提取物显著激活凝血酶诱导的血小板黏附（105.27 ± 0.11%，P < 0.05）。没有一种提取物能改善血小板聚集。对氯仿提取物的进一步GC - MS分析揭示了五种具有潜在血小板激活特性的关键植物化学成分。总之，我们的研究评估了克拉东博克对血小板激活以及潜在的伤口愈合特性。GC - MS分析确定了克拉东博克中潜在的生物活性植物化学化合物，这些化合物值得进一步研究以表征其特性。
