College of Landscape Architecture and Forestry, Qingdao Agricultural University, Qingdao, 266000, China.
College of Landscape, Beijing Forestry University, Beijing, 100083, China.
Plant Cell Rep. 2022 Feb;41(2):395-413. doi: 10.1007/s00299-021-02815-0. Epub 2021 Nov 24.
Nine RcBURPs have been identified in Rosa chinensis, and overexpression of RcBURP4 increased ABA, NaCl sensitivity, and drought tolerance in transgenic Arabidopsis. BURP proteins are unique to plants and may contribute greatly to growth, development, and stress responses of plants. Despite the vital role of BURP proteins, little is known about these proteins in rose (Rosa spp.). In the present study, nine genes belonging to the BURP family in R. chinensis were identified using multiple bioinformatic approaches against the rose genome database. The nine RcBURPs, with diverse structures, were located on all chromosomes of the rose genome, except for Chr2 and Chr3. Phylogenic analysis revealed that these RcBURPs can be classified into eight subfamilies, including BNM2-like, PG1β-like, USP-like, RD22-like, BURP-V, BURP-VI, BURP-VII, and BURP-VIII. Conserved motif and exon-intron analyses indicated a conserved pattern within the same subfamily. The presumed cis-regulatory elements (CREs) within the promoter region of each RcBURP were analyzed and the results showed that all RcBURPs contained different types of CREs, including abiotic stress-, light response-, phytohormones response-, and plant growth and development-related CREs. Transcriptomic analysis revealed that a BURP-V member, RcBURP4, was induced in rose leaves and roots under mild and severe drought treatments. We then overexpressed RcBURP4 in Arabidopsis and examined its role under abscisic acid (ABA), NaCl, polyethylene glycol (PEG), and drought treatments. Nine stress-responsive genes expression were changed in RcBURP4-overexpressing leaves and roots. Furthermore, RcBURP4-silenced rose plants exhibited decreased tolerance to dehydration. The results obtained from this study provide the first comprehensive overview of RcBURPs and highlight the importance of RcBURP4 in rose plant.
已在月季中鉴定出 9 个 RcBURP，过表达 RcBURP4 增加了转基因拟南芥中的 ABA、NaCl 敏感性和耐旱性。BURP 蛋白是植物特有的，可能对植物的生长、发育和应激反应有很大贡献。尽管 BURP 蛋白具有重要作用，但对玫瑰（Rosa spp.）中的这些蛋白知之甚少。本研究采用多种生物信息学方法，针对玫瑰基因组数据库，鉴定了 9 个属于 R. chinensis 的 BURP 家族基因。这 9 个 RcBURPs 结构多样，位于玫瑰基因组的所有染色体上，除了 Chr2 和 Chr3。系统发育分析表明，这些 RcBURPs 可分为 8 个亚家族，包括 BNM2 样、PG1β 样、USP 样、RD22 样、BURP-V、BURP-VI、BURP-VII 和 BURP-VIII。保守基序和外显子-内含子分析表明同一亚家族内具有保守模式。分析了每个 RcBURP 启动子区域内的假定顺式调控元件（CREs），结果表明，所有 RcBURPs 均包含不同类型的 CREs，包括非生物胁迫、光响应、植物激素响应和植物生长发育相关的 CREs。转录组分析表明，BURP-V 成员 RcBURP4 在玫瑰叶片和根中受到轻度和重度干旱处理诱导。随后，我们在拟南芥中过表达 RcBURP4，并在 ABA、NaCl、聚乙二醇（PEG）和干旱处理下研究其作用。RcBURP4 过表达叶片和根中的 9 个应激响应基因表达发生改变。此外，沉默 RcBURP4 的玫瑰植物对脱水的耐受性降低。本研究结果首次全面概述了 RcBURPs，并强调了 RcBURP4 在玫瑰植物中的重要性。
