College of Landscape Architecture and Forestry, Qingdao Agricultural University, Qingdao, 266000, China.
Shandong Provincial Center of Forest Tree Germplasm Resources, Jinan, 250102, China.
Planta. 2021 Nov 10;254(6):118. doi: 10.1007/s00425-021-03778-y.
A total of 27 rose thaumatin-like protein (TLP) genes were identified from the rose genome through bioinformatics analyses. RcTLP6 was found to confer salinity stress tolerance in rose. Thaumatin-like proteins (TLPs) play critical roles in regulating many biological processes, including abiotic and biotic stress responses in plants. Here, we conducted a genome-wide screen of TLPs in rose (Rosa chinensis) and identified 27 RcTLPs. The identified RcTLPs, as well as other TLPs from six different plant species, were placed into nine groups based on a phylogenetic analysis. An analysis of the intron-exon structures of the TLPs revealed a high degree of similarity. RcTLP genes were found on all chromosomes except for chromosome four. Cis-regulatory elements (CEs) were identified in the promoters of all RcTLPs, including CEs associated with growth, development and hormone-responsiveness, as well as abiotic and biotic responses, indicating they play diverse roles in rose. Transcriptomics analysis revealed that RcTLPs had tissue-specific expression patterns, and several root-preferential RcTLPs were responsive to drought and salinity stress. Quantitative PCR analysis of six RcTLPs under ABA, PEG and NaCl treatment confirmed the differentially expressed genes identified in the transcriptomics experiment. In addition, silencing RcTLP6 in rose leaves led to decreased tolerance to salinity stress. We also screened proteins which may interact with RcTLP6 to understand its biological roles. This study represents the first report of the TLP gene family in rose and expands the current understanding of the role that RcTLP6 plays in salt tolerance. These findings lay a foundation for future utilization of RcTLPs to improve rose abiotic stress tolerance.
通过生物信息学分析，从玫瑰基因组中鉴定出 27 个玫瑰硫氧还蛋白样蛋白（TLP）基因。发现 RcTLP6 赋予玫瑰耐盐性。硫氧还蛋白样蛋白（TLPs）在植物的非生物和生物胁迫反应等许多生物学过程中发挥着关键作用。在这里，我们对玫瑰（Rosa chinensis）中的 TLPs 进行了全基因组筛选，鉴定出 27 个 RcTLPs。根据系统发育分析，将鉴定出的 RcTLPs 以及来自其他 6 种不同植物的其他 TLPs 分为 9 组。TLPs 基因的内含子-外显子结构分析表明其具有高度相似性。除了染色体四之外，RcTLP 基因存在于所有染色体上。所有 RcTLP 的启动子中都鉴定出顺式作用元件（CEs），包括与生长、发育和激素响应以及非生物和生物响应相关的 CEs，表明它们在玫瑰中发挥着多种作用。转录组学分析表明，RcTLPs 具有组织特异性表达模式，并且一些根偏好的 RcTLPs 对干旱和盐胁迫有反应。ABA、PEG 和 NaCl 处理下 6 个 RcTLPs 的定量 PCR 分析证实了转录组实验中鉴定出的差异表达基因。此外，沉默玫瑰叶片中的 RcTLP6 会导致耐盐性降低。我们还筛选了可能与 RcTLP6 相互作用的蛋白质，以了解其生物学作用。这项研究代表了玫瑰中 TLP 基因家族的首次报道，并扩展了对 RcTLP6 在耐盐性中作用的现有认识。这些发现为未来利用 RcTLPs 提高玫瑰的非生物胁迫耐受性奠定了基础。
