长链非编码RNA LINC01559通过增强肺腺癌中的自噬发挥致癌作用。

Long non-coding RNA LINC01559 exerts oncogenic role via enhancing autophagy in lung adenocarcinoma.

作者信息

Zhao Zhuochen, Wan Junhu, Guo Manman, Yang Zhengwu, Li Zhuofang, Wang Yangxia, Ming Liang

机构信息

Department of Clinical Laboratory, The First Affiliated Hospital of Zhengzhou University and the Key Clinical Laboratory of Henan Province, Henan, China.

出版信息

Cancer Cell Int. 2021 Nov 25;21(1):624. doi: 10.1186/s12935-021-02338-4.

BACKGROUND

Long non-coding RNAs (lncRNAs) have been verified to play fatal role in regulating the progression of lung adenocarcinoma (LUAD). Although lncRNAs play important role in regulating the autophagy of tumor cells, the function and molecular mechanism of LINC01559 in regulating lung cancer development remain to be elucidated.

METHOD AND MATERIALS

In this study, we used bioinformatics to screen out autophagy-related lncRNAs from TCGA-LUAD repository. Then the least absolute shrinkage and selection operator (LASSO) regression was applied to establish the signature of autophagy-related lncRNAs so that clinical characteristics and survival in LUAD patients be evaluated. Finally, we selected the most significant differences lncRNA, LINC01559, to verify its function in regulating LUAD progression in vitro.

RESULTS

We found high expression of LINC01559 indicates lymph node metastasis and poor prognosis. Besides, LINC01559 promotes lung cancer cell proliferation and migration in vitro, by enhancing autophagy signal pathway via sponging hsa-miR-1343-3p.

CONCLUSION

We revealed a novel prognostic model based on autophagy-related lncRNAs, and provide a new therapeutic target and for patients with lung adenocarcinoma named LINC01559.

背景

长链非编码RNA（lncRNAs）已被证实对肺腺癌（LUAD）进展具有重要调控作用。尽管lncRNAs在肿瘤细胞自噬调控中发挥重要作用，但LINC01559在肺癌发生发展中的功能及分子机制仍有待阐明。

方法与材料

本研究中，我们利用生物信息学从TCGA-LUAD数据库中筛选出与自噬相关的lncRNAs。随后应用最小绝对收缩和选择算子（LASSO）回归建立自噬相关lncRNAs特征，进而评估LUAD患者的临床特征和生存情况。最后，我们选取差异最显著的lncRNA即LINC01559，在体外验证其对LUAD进展的调控作用。