Structure of KOH-extractable polysaccharides of tree greenery of from Siberian fir Abies sibirica Ledeb.

Polysaccharide AS was extracted with aqueous KOH from the Abies sibirica foliage. Pectin, xylan, AGPs and xyloglucan were isolated from AS by anion-exchange chromatography and Smith degradation, combined with enzymatic cleavage. Potential interactions between those polymers were examined. Since xylan, AGPs, RG-I and glucan were co-fractionated following complete removal of galacturonan (HG) with polygalacturonase, it can be inferred that the enzymatic cleavage of HG did not affect co-fractionation of cross-linking glycans, RG-I and AGPs. These were hypothesized to be bound via a covalent cross-link, as the removal of HG regions did not affect this complex. Given that pectin was represented solely by RG-I regions, it can be assumed that some of glucan, xylan and AGPs were associated directly with RG-I. Besides, the enzymatic hydrolysis eliminated most of xylose and rhamnose. This suggests implicitly that some of xylan was linked to RG-I.