Shakhmatov Evgeny G, Makarova Elena N
Institute of Chemistry, Federal Research Center "Komi Science Center of the Ural Branch of the Russian Academy of Sciences", Pervomaiskaya St. 48, Syktyvkar 167982, Russia.
Institute of Chemistry, Federal Research Center "Komi Science Center of the Ural Branch of the Russian Academy of Sciences", Pervomaiskaya St. 48, Syktyvkar 167982, Russia.
Carbohydr Polym. 2022 Jan 15;276:118794. doi: 10.1016/j.carbpol.2021.118794. Epub 2021 Oct 20.
Polysaccharide AS was extracted with aqueous KOH from the Abies sibirica foliage. Pectin, xylan, AGPs and xyloglucan were isolated from AS by anion-exchange chromatography and Smith degradation, combined with enzymatic cleavage. Potential interactions between those polymers were examined. Since xylan, AGPs, RG-I and glucan were co-fractionated following complete removal of galacturonan (HG) with polygalacturonase, it can be inferred that the enzymatic cleavage of HG did not affect co-fractionation of cross-linking glycans, RG-I and AGPs. These were hypothesized to be bound via a covalent cross-link, as the removal of HG regions did not affect this complex. Given that pectin was represented solely by RG-I regions, it can be assumed that some of glucan, xylan and AGPs were associated directly with RG-I. Besides, the enzymatic hydrolysis eliminated most of xylose and rhamnose. This suggests implicitly that some of xylan was linked to RG-I.
用氢氧化钾水溶液从西伯利亚冷杉树叶中提取多糖AS。通过阴离子交换色谱法、史密斯降解法并结合酶解从AS中分离出果胶、木聚糖、阿拉伯半乳聚糖蛋白（AGPs）和木葡聚糖。研究了这些聚合物之间的潜在相互作用。由于用多聚半乳糖醛酸酶完全去除半乳糖醛酸（HG）后，木聚糖、AGPs、鼠李糖半乳糖醛酸聚糖-I（RG-I）和葡聚糖会一起被分离出来，因此可以推断HG的酶解不会影响交联聚糖、RG-I和AGPs的共分离。由于去除HG区域不会影响这种复合物，因此推测它们是通过共价交联结合在一起的。鉴于果胶仅由RG-I区域代表，可以假设一些葡聚糖、木聚糖和AGPs直接与RG-I相关联。此外，酶水解消除了大部分木糖和鼠李糖。这暗示着一些木聚糖与RG-I相连。
