Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran.
Department of Pathobiology, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran.
Arch Razi Inst. 2021 Sep 1;76(3):445-452. doi: 10.22092/ari.2020.128449.1415. eCollection 2021 Summer.
Brucellosis is an anthropozoonotic disease. Infection of livestock with is endemic in most parts of Iran. Sistan-Baluchestan is bordered on the east by the countries of Afghanistan and Pakistan. The high prevalence of brucellosis in livestock in the eastern neighboring countries results in transmission of the disease to this province. The present research aimed to determine the prevalence of brucellosis in small ruminants in the Sistan region of Iran and to compare serological and molecular tests for the detection of brucellosis. Blood samples were taken from 150 randomly selected sheep and goats, and sera were separated. All sera were analyzed by serological (Wright and 2-ME) and molecular (Polymerase Chain Reaction (PCR)) tests. Serological tests were carried out according to the instructions of the Iranian Veterinary Organization The degree of agreement between serological tests and PCR was determined by kappa value. In this study, 17 cases (11.3%) were identified as positive by the PCR method. Wright and 2-ME tests had the highest agreement with PCR in titers ≥2/80 and ≥2/40, respectively. The results of this study show that the brucellosis in sheep and goats has a greater prevalence in the Sistan region than in most other parts of Iran, and this is important in terms of public health. It is suggested that brucellosis vaccination coverage in livestock be increased in this area and that the people in Sistan region must be notified about methods for preventing brucellosis. Also, further studies to compare conventional serologic tests with the gold standard test are recommended.
布鲁氏菌病是一种人畜共患病。在伊朗的大部分地区，家畜感染 呈地方性流行。锡斯坦和俾路支斯坦与阿富汗和巴基斯坦接壤。邻国家畜布鲁氏菌病的高流行率导致该疾病传播到该省。本研究旨在确定伊朗锡斯坦地区小反刍动物布鲁氏菌病的流行率，并比较血清学和分子检测方法用于检测布鲁氏菌病。从 150 只随机选择的绵羊和山羊中采集血液样本，并分离血清。所有血清均通过血清学（Wright 和 2-ME）和分子（聚合酶链反应（PCR））检测进行分析。血清学检测按照伊朗兽医组织的说明进行。通过 Kappa 值确定血清学检测与 PCR 之间的一致性程度。在这项研究中，17 例（11.3%）通过 PCR 方法被确定为阳性。在滴度≥2/80 和≥2/40 时，Wright 和 2-ME 试验与 PCR 的一致性最高。本研究结果表明，绵羊和山羊的布鲁氏菌病在锡斯坦地区的流行率高于伊朗的大多数其他地区，这在公共卫生方面很重要。建议增加该地区家畜的布鲁氏菌病疫苗接种覆盖率，并通知锡斯坦地区的人们预防布鲁氏菌病的方法。还建议进一步研究将常规血清学检测与金标准检测进行比较。
