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桔梗白色和蓝色花朵的比较转录组分析，以阐明参与花青素生物合成的基因

Comparative Transcriptomic Analysis on White and Blue Flowers of Platycodon grandiflorus to Elucidate Genes Involved in the Biosynthesis of Anthocyanins.

作者信息

Lv Yanping, Tong Xiaohui, Zhang Pengfei, Yu Nianjun, Gui Shuangying, Han Rongchun, Ge Dezhu

机构信息

School of Pharmacy, Anhui University of Chinese Medicine; Hefei 230012, China.

School of Life Sciences, Anhui University of Chinese Medicine; Hefei 230012, China.

出版信息

Iran J Biotechnol. 2021 Jul 1;19(3):e2811. doi: 10.30498/ijb.2021.239899.2811. eCollection 2021 Jul.

DOI:10.30498/ijb.2021.239899.2811
PMID:34825015
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8590723/
Abstract

BACKGROUND

has long been used in Northeast Asia as a food and folk medicine to treat various diseases. The intense blue color of corolla is its characteristic feature.

OBJECTIVES

By comparing deep transcriptomic data of and its white cultivar, we intended to elucidate the molecular mechanisms concerning the biosynthesis of anthocyanins in this plant.

MATERIAL AND METHODS

We sampled blue mature flowers (PgB) and yellow young buds (PgY) of . Meanwhile, mature flowers (PgW) of white cultivar were also collected for RNA extraction and next-generation sequencing. After high-throughput sequencing, Trinity software was applied for de novo assembly and the resultant 49934 unigenes were subjected for expression analysis and annotation against NR, KEGG, UniProt, and Pfam databases.

RESULTS

In all, 32.77 Gb raw data were generated and the gene expression profile for the flowers of was constructed. Pathway enrichment analysis demonstrated that genes involved in flavone and flavonol biosynthesis were differently expressed.

CONCLUSIONS

The extremely low expression of flavonoid-3',5'-hydroxylase in PgY and PgW was regarded as the reason for the formation of its white cultivar. Our findings provided useful information for further studies into the biosynthetic mechanism of anthocyanins.

摘要

背景

长期以来在东北亚地区被用作食物和民间药物来治疗各种疾病。其花冠强烈的蓝色是其特征。

目的

通过比较该植物及其白色栽培品种的深度转录组数据，我们旨在阐明该植物中花青素生物合成的分子机制。

材料与方法

我们采集了该植物的蓝色成熟花（PgB）和黄色幼芽（PgY）。同时，也采集了该植物白色栽培品种的成熟花（PgW）用于RNA提取和下一代测序。高通量测序后，使用Trinity软件进行从头组装，得到的49934个单基因进行表达分析，并与NR、KEGG、UniProt和Pfam数据库进行注释。

结果

总共产生了32.77 Gb的原始数据，并构建了该植物花的基因表达谱。通路富集分析表明，参与黄酮和黄酮醇生物合成的基因表达存在差异。

结论

PgY和PgW中黄酮-3',5'-羟化酶的极低表达被认为是其白色栽培品种形成的原因。我们的研究结果为进一步研究花青素的生物合成机制提供了有用信息。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18b3/8590723/52367320969b/IJB-19-e2811-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18b3/8590723/a6ab84dcce52/IJB-19-e2811-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18b3/8590723/a0981f0b3a91/IJB-19-e2811-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18b3/8590723/ec74898af17e/IJB-19-e2811-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18b3/8590723/ec08e8cefe03/IJB-19-e2811-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18b3/8590723/52367320969b/IJB-19-e2811-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18b3/8590723/a6ab84dcce52/IJB-19-e2811-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18b3/8590723/a0981f0b3a91/IJB-19-e2811-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18b3/8590723/ec74898af17e/IJB-19-e2811-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18b3/8590723/ec08e8cefe03/IJB-19-e2811-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18b3/8590723/52367320969b/IJB-19-e2811-g005.jpg

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