Kim Eunhui, Hyun Tae Kyung
Department of Industrial Plant Science and Technology, College of Agricultural, Life and Environmental Sciences, Chungbuk National University, Cheongju, 28644 Republic of Korea.
3 Biotech. 2023 Mar;13(3):75. doi: 10.1007/s13205-023-03490-6. Epub 2023 Feb 3.
Floral color plays a major role in pollinator specificity, and changes in color may result in pollinator shifts and pollinator-mediated speciation. In the purple flowers of , anthocyanins are the major pigment metabolites, whereas white flowers result due to the absence of anthocyanins. The lack of anthocyanins may be due to the inhibition of the anthocyanin biosynthesis pathway. However, the molecular mechanism of anthocyanin biosynthesis in is not fully understood. Hence, we identified R2R3-MYB transcription factor, PlgMYBR1, as a negative regulator for anthocyanin biosynthesis using sequence homology and tissue-specific expression pattern analyses. A heterologous co-expression assay suggested that PlgMYBR1 inhibited the function of AtPAP1 ( production of anthocyanin pigment 1), indicating that PlgMYBR1 plays as a repressor of anthocyanin biosynthesis in . Our results provide a foundation for future efforts to understand the anthocyanin biosynthesis in and, thereby, to improve flower color through genetic engineering.
The online version contains supplementary material available at 10.1007/s13205-023-03490-6.
花色在传粉者特异性中起主要作用，颜色变化可能导致传粉者转变和传粉者介导的物种形成。在[植物名称]的紫色花朵中，花青素是主要的色素代谢产物，而白色花朵是由于缺乏花青素所致。花青素的缺乏可能是由于花青素生物合成途径受到抑制。然而，[植物名称]中花青素生物合成的分子机制尚未完全了解。因此，我们通过序列同源性和组织特异性表达模式分析，鉴定出R2R3-MYB转录因子PlgMYBR1是花青素生物合成的负调控因子。异源共表达试验表明，PlgMYBR1抑制AtPAP1（花青素色素产生1）的功能，表明PlgMYBR1在[植物名称]中作为花青素生物合成的阻遏物发挥作用。我们的结果为未来了解[植物名称]中花青素生物合成、从而通过基因工程改善花色的研究奠定了基础。
在线版本包含可在10.1007/s13205-023-03490-6获取的补充材料。
