Frontiers Science Center for Molecular Design Breeding, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, 100193, China.
Tongfang Knowledge Network Digital Publishing Technology Co., LTD, Taiyuan, 030006, Shanxi, China.
Theor Appl Genet. 2024 Aug 13;137(9):203. doi: 10.1007/s00122-024-04706-w.
The common wheat line 4N0461 showed adult-plant resistance to leaf rust. 4N0461 was crossed with susceptible cultivars Nongda4503 and Shi4185 to map the causal resistance gene(s). Segregation of leaf rust response in F populations from both crosses was 9 resistant:7 susceptible, indicative of two complementary dominant resistance genes. The genes were located on chromosome arms 3BS and 4BL and temporarily named LrN3B and LrN4B, respectively. Subpopulations from 4N0461 × Nongda4503 with LrN3B segregating as a single allele were used to fine-map LrN3B locus. LrN3B was delineated in a genetic interval of 0.07 cM, corresponding to 106 kb based on the Chinese Spring reference genome (IWGSC RefSeq v1.1). Four genes were annotated in this region, among which TraesCS3B02G014800 and TraesCS3B02G014900 differed between resistant and susceptible genotypes, and both were required for LrN3B resistance in virus-induced gene silencing experiments. Diagnostic markers developed for checking the polymorphism of each candidate gene, can be used for marker-assisted selection in wheat breeding programs.
普通小麦品系 4N0461 表现出成株期抗叶锈病。将 4N0461 与感病品种 Nongda4503 和 Shi4185 杂交，以定位导致抗性的基因。来自两个杂交的 F 群体的叶锈病反应的分离为 9 抗：7 感，表明存在两个互补的显性抗性基因。这些基因位于 3BS 和 4BL 染色体臂上，分别暂时命名为 LrN3B 和 LrN4B。来自 4N0461×Nongda4503 的具有作为单个等位基因分离的 LrN3B 的亚群体用于精细定位 LrN3B 基因座。LrN3B 被描绘在 0.07 cM 的遗传间隔内，基于中国春参考基因组（IWGSC RefSeq v1.1），对应于 106 kb。在该区域注释了四个基因，其中 TraesCS3B02G014800 和 TraesCS3B02G014900 在抗性和感病基因型之间存在差异，并且在病毒诱导的基因沉默实验中都需要 LrN3B 抗性。为检查每个候选基因的多态性而开发的诊断标记，可以用于小麦育种计划中的标记辅助选择。
