Hirose Mina, Aung Meiji Soe, Fujita Yusuke, Kato Taisei, Hirose Yukito, Yahata Shoko, Fukuda Atsushi, Saitoh Masato, Urushibara Noriko, Kobayashi Nobumichi
Division of Pediatric Dentistry, Department of Oral Growth and Development, School of Dentistry, Health Sciences University of Hokkaido, Ishikari-Tobetsu 061-0293, Japan.
Department of Hygiene, Sapporo Medical University School of Medicine, Sapporo 060-8556, Japan.
Pathogens. 2022 Jul 28;11(8):849. doi: 10.3390/pathogens11080849.
The spread of methicillin resistance and virulence among staphylococci in the community poses a public health concern. In this study, we investigated the prevalence of species colonizing the oral cavity and hand (skin) of healthy university students and their phenotypic and genetic characteristics in northern Japan. Among a total of 332 subjects, 6 and 110 methicillin-resistant and susceptible (MRSA and MSSA, respectively) isolates were recovered from 105 subjects. MRSA isolates were genotyped as CC5, CC8, CC45, and CC59 with SCC-IIa or IV, among which an isolate of ST6562 (single-locus variant of ST8) harbored SCC-IVa, PVL genes and ACME-I, which are the same traits as the USA300 clone. ST1223 was isolated from the oral cavity and hand of a single student. Coagulase-negative (CoNS) was recovered from 154 subjects (172 isolates), and classified into 17 species, with being the most common (38%), followed by (24%) and (15%), including nine -positive isolates. was differentiated into seven clusters/subclusters, and genetic factors associated with the NRCS-A clone (, , ) were detected in 10-21% of isolates. The colonization of the USA300-like MRSA variant and with the traits of the NRCS-A clone in healthy individuals was noteworthy.
甲氧西林耐药性和葡萄球菌毒力在社区中的传播引起了公共卫生关注。在本研究中，我们调查了日本北部健康大学生口腔和手部（皮肤）定植菌的流行情况及其表型和基因特征。在总共332名受试者中，从105名受试者中分离出6株耐甲氧西林和110株敏感葡萄球菌（分别为MRSA和MSSA）。MRSA分离株的基因型为CC5、CC8、CC45和CC59，带有SCC-IIa或IV，其中一株ST6562（ST8的单基因座变体）携带SCC-IVa、PVL基因和ACME-I，这些特征与USA300克隆相同。ST1223是从一名学生的口腔和手部分离出来的。凝固酶阴性葡萄球菌（CoNS）从154名受试者（172株分离株）中分离出来，分为17个种，其中表皮葡萄球菌最常见（38%），其次是溶血葡萄球菌（24%）和头状葡萄球菌（15%），包括9株阳性分离株。表皮葡萄球菌分为7个簇/亚簇，在10%-21%的分离株中检测到与NRCS-A克隆（icaA、icaD、icaR）相关的遗传因素。健康个体中类似USA300的MRSA变体和具有NRCS-A克隆特征的表皮葡萄球菌的定植情况值得关注。
