Abuoghaba Ahmed Abdel-Kareem, Ragab Mona A, Shazly Soheir A, Kokoszyński Dariusz, Saleh Mohamed
Department of Poultry Production, Faculty of Agriculture, Sohag University, Sohag 82524, Egypt.
Agricultural Research Center, Institute of Animal Production Research, Ministry of Agriculture, Dokki, Giza 12618, Egypt.
Animals (Basel). 2021 Nov 11;11(11):3220. doi: 10.3390/ani11113220.
This study evaluated the impact of incubation temperature and spraying hatching eggs with curcumin during the early embryogenesis phase on chick embryo developments, hatchability, physiological body reactions, and hormonal profiles of Dokki 4 chickens. A total of 720 fertile eggs were equally distributed into two groups. In the first group, the eggs were incubated at normal incubation temperature/NIT (37.8 °C and 55-60% RH) for up to 19 days of incubation, whereas those in the second group were incubated in the same conditions except from 6 to 8 day, in which they were daily exposed to chronic incubation temperature/CIT (39.0 °C) for 3 h. Each group was classified into four curcumin treatment doses; the 1st treatment (control) was sprayed with distilled water, while the 2nd, 3rd, and 4th treatments were sprayed with 250, 500, and 1000 mg curcumin/liter distilled water. The results indicated that the lowest hatchability of fertile eggs (%) was obtained in the CIT group ( = 0.02), whereas the highest body surface temperature/BST compared in the NIT group ( = 0.01). Regarding curcumin treatments, the percentages of heart, gizzard, spleen, and T3 hormone levels in the treated group were significantly increased, while the H/L ratio was significantly reduced ( = 0.001) compared with the control. At 8 weeks of age, the testes and ovary percentages in treated groups were significantly ( = 0.05) increased compared with the control. In conclusion, exposure of hatching eggs to high thermal stress (39 °C) during the incubation phase had deleterious effects on chick performance and T3 hormone level. Moreover, spraying hatching eggs had beneficial impacts on growth, reproductive organs, T3 hormone level, and reducing H/L ratio.
本研究评估了孵化温度以及在胚胎发育早期用姜黄素喷洒孵化蛋对多基4号鸡胚胎发育、孵化率、生理机体反应和激素水平的影响。总共720枚受精蛋被平均分为两组。第一组蛋在正常孵化温度/正常孵化温度（NIT，37.8°C和55 - 60%相对湿度）下孵化至19天，而第二组蛋在相同条件下孵化，只是在第6至8天每天暴露于慢性孵化温度/慢性孵化温度（CIT，39.0°C）下3小时。每组又分为四个姜黄素处理剂量组；第一个处理组（对照组）喷洒蒸馏水，而第二、第三和第四个处理组分别喷洒250、500和1000毫克/升蒸馏水的姜黄素溶液。结果表明，CIT组的受精蛋孵化率最低（P = 0.02），而NIT组的体表温度最高（P = 0.01）。关于姜黄素处理，与对照组相比，处理组的心脏、砂囊、脾脏和T3激素水平百分比显著增加，而H/L比值显著降低（P = 0.001）。在8周龄时，与对照组相比，处理组的睾丸和卵巢百分比显著增加（P = 0.05）。总之，在孵化阶段将孵化蛋暴露于高热应激（39°C）对雏鸡性能和T3激素水平有有害影响。此外，用姜黄素喷洒孵化蛋对生长、生殖器官、T3激素水平以及降低H/L比值有有益影响。
