Guo Fanghua, Tsao Rong, Li Chuyao, Wang Xiaoya, Zhang Hua, Jiang Li, Sun Yong, Xiong Hua
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
Guelph Research and Development Centre, Agricultural and Agri-Food Canada, 93 Stone Road West, Guelph, ON N1G 5C9, Canada.
Foods. 2021 Nov 11;10(11):2765. doi: 10.3390/foods10112765.
As a processing by-product, green pea hull (GPH) was found to be rich in phenolic components in our previous studies. In this study, UHPLC-LTQ-OrbiTrap-MS (Ultra performance liquid chromatography-linear ion trap orbitrap tandem mass spectrometry) technique was used to quantify polyphenols, and DSS (sodium dextran sulfate)-induced colitis mouse model was established to explore the effect of GPH extracts on colitis. The results showed that quercetin and its derivatives, kaempferol trihexanside and catechin and its derivatives were the main phenolic substances in the extract, reaching 2836.57, 1482.00 and 1339.91 µg quercetin/g GPH extract, respectively; GPH extracts can improved inflammatory status, repaired colonic function, regulated inflammatory factors, and restored oxidative balance in mice. Further, GPH extracts can activate Keap1-Nrf2-ARE signaling pathway, regulate downstream antioxidant protease and gut microbiota by increasing F/B value and promoting the growth of and , and improve the level of SCFAs (short-chain fatty acids) to relieve DSS-induced colitis in mice. Therefore, GPH may be a promising dietary resource for the treatment of ulcerative colitis.
在我们之前的研究中，发现绿豌豆壳（GPH）作为一种加工副产品富含酚类成分。在本研究中，采用超高效液相色谱-线性离子阱轨道阱串联质谱（UHPLC-LTQ-OrbiTrap-MS）技术对多酚进行定量，并建立葡聚糖硫酸钠（DSS）诱导的结肠炎小鼠模型，以探究GPH提取物对结肠炎的影响。结果表明，槲皮素及其衍生物、山奈酚三己糖苷、儿茶素及其衍生物是提取物中的主要酚类物质，分别达到2836.57、1482.00和1339.91 μg槲皮素/g GPH提取物；GPH提取物可改善小鼠的炎症状态，修复结肠功能，调节炎症因子，并恢复氧化平衡。此外，GPH提取物可激活Keap1-Nrf2-ARE信号通路，通过增加F/B值和促进 和 的生长来调节下游抗氧化蛋白酶和肠道微生物群，并提高短链脂肪酸（SCFA）水平，以缓解DSS诱导的小鼠结肠炎。因此，GPH可能是一种有前景的治疗溃疡性结肠炎的膳食资源。
