Sinan Kouadio Ibrahime, Zengin Gokhan, Zheleva-Dimitrova Dimitrina, Gevrenova Reneta, Picot-Allain Marie Carene Nancy, Dall'Acqua Stefano, Behl Tapan, Goh Bey Hing, Ying Patrick Tang Siah, Mahomoodally Mohamad Fawzi
Biochemistry and Physiology Research Laboratory, Department of Biology, Science Faculty, Selcuk University, Konya 42130, Turkey.
Department of Pharmacognosy, Faculty of Pharmacy, Medical University-Sofia, 2 Dunav Str., 1000 Sofia, Bulgaria.
Antioxidants (Basel). 2021 Nov 5;10(11):1771. doi: 10.3390/antiox10111771.
species have been used in traditional medicine for different human ailments. In this study, the effect of different solvents (ethyl acetate, methanol, and water) and extraction methods (infusion, maceration, and Soxhlet extraction) on the enzyme inhibitory activity against acetylcholinesterase, butyrylcholinesterase, tyrosinase, α-amylase, α-glucosidase, and antioxidant properties of and leaves and stem bark were evaluated. Ultra-high-performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMS) yield in the identification and/or annotation of 98 compounds showing that the main secondary metabolites of the plant are gallic and ellagic acids and their derivatives, ellagitannins, hydroxybenzoic, hydroxycinnamic, acylquinic acids and flavonols, flavanones, and flavanonols. The leaves infusion of both species showed highest inhibition against acetylcholinesterase (AChE) (10.10 and 10.45 mg galantamine equivalent (GALAE)/g, for and , respectively). The ethyl acetate extracts of the stem bark of and actively inhibited α-glucosidase. Methanolic extracts of the leaves and stem bark exhibited highest tyrosinase inhibitory action. Antioxidant activity and higher levels of phenolics were observed for the methanolic extracts of The results suggested that the species could be considered as natural phyto-therapeutic agents in medicinal and cosmeceutical applications.
不同物种已被用于传统医学治疗各种人类疾病。在本研究中，评估了不同溶剂（乙酸乙酯、甲醇和水）和提取方法（浸泡、浸渍和索氏提取）对[具体物种1]和[具体物种2]的叶、茎皮针对乙酰胆碱酯酶、丁酰胆碱酯酶、酪氨酸酶、α-淀粉酶、α-葡萄糖苷酶的酶抑制活性以及抗氧化性能的影响。超高效液相色谱-高分辨率质谱（UHPLC-HRMS）鉴定和/或注释出98种化合物，表明该植物的主要次生代谢产物是没食子酸和鞣花酸及其衍生物、鞣花单宁、羟基苯甲酸、羟基肉桂酸、酰基奎宁酸以及黄酮醇、黄烷酮和二氢黄酮醇。两种[具体物种]的叶浸液对乙酰胆碱酯酶（AChE）的抑制作用最强（[具体物种1]和[具体物种2]分别为10.10和10.45 mg加兰他敏当量（GALAE）/g）。[具体物种1]和[具体物种2]茎皮的乙酸乙酯提取物对α-葡萄糖苷酶有显著抑制作用。叶和茎皮的甲醇提取物表现出最强的酪氨酸酶抑制作用。[具体物种1]的甲醇提取物具有抗氧化活性且酚类物质含量较高。结果表明，[具体物种]可被视为医药和化妆品应用中的天然植物治疗剂。
