Graduate School of Biotechnology, Kyung Hee University, Seocheon, Giheung, Yongin 446-701, Gyeonggi-do, Korea.
Department of Genetic Engineering, College of Life Science, Graduate School of Biotechnology, Kyung Hee University, Seocheon, Giheung, Yongin 446-701, Gyeonggi-do, Korea.
Int J Mol Sci. 2021 Nov 21;22(22):12523. doi: 10.3390/ijms222212523.
(1) Background: is the most common cause of fungal infections worldwide, but due to the limited option of antifungal therapies, alternative strategies are required. (2) Methods: var. extract was used for the biofilm formation assay using RPMI1640. The combinatorial antifungal assay, the dimorphic transition assay, and the adherence assay were done to see the influence of inhibition of biofilm formation. qRT-PCR analysis were performed to check the gene expression. (3) Results: var. extract inhibited the biofilm formation. Treatment of extract increased the antifungal susceptibility of miconazole from a 37% reduction in fungal growth to 99.05%, and also dose-dependently reduced the dimorphic transition of and the attachment of to HaCaT cells. The extract blocked the expression of hyphal-related genes, extracellular matrix genes, Ras1-cAMP-PKA pathway genes, Cph2-Tec1 pathway gene, and MAP kinase pathway gene. (4) Conclusions: In this study, the treatment of var extract showed inhibition of fungal biofilm formation, activation of antifungal susceptibility, and reduction of infection. These results suggest that fungal biofilm formation is a good target for the development of antifungal adjuvants, and var extract should be a good candidate for biofilm-associated fungal infections.
(1) 背景： 是全球最常见的真菌感染原因，但由于抗真菌治疗选择有限，需要寻找替代策略。(2) 方法：使用 RPMI1640 进行 var. 提取物的生物膜形成测定。进行组合抗真菌测定、二态性转变测定和黏附测定，以观察抑制生物膜形成的影响。进行 qRT-PCR 分析以检查基因表达。(3) 结果： var. 提取物抑制了 生物膜形成。提取物处理增加了咪康唑的抗真菌敏感性，使真菌生长减少了 37%至 99.05%，并呈剂量依赖性降低了 和 向 HaCaT 细胞的附着。提取物阻断了菌丝相关基因、细胞外基质基因、Ras1-cAMP-PKA 途径基因、Cph2-Tec1 途径基因和 MAP 激酶途径基因的表达。(4) 结论：在这项研究中， var 提取物的处理显示出抑制真菌生物膜形成、激活抗真菌敏感性和减少感染的作用。这些结果表明，真菌生物膜形成是开发抗真菌佐剂的一个很好的靶点，而 var 提取物应该是与生物膜相关的真菌感染的一个很好的候选物。
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