School of Nutrition and Health Sciences, Taipei Medical University, Taipei 11031, Taiwan.
Research Center of Geriatric Nutrition, College of Nutrition, Taipei Medical University, Taipei 11031, Taiwan.
Int J Mol Sci. 2021 Nov 21;22(22):12547. doi: 10.3390/ijms222212547.
The purpose of this study was to investigate the beneficial effects of synbiotics on liver damage, intestinal health, and muscle loss, and their relevance in rats with chronic ethanol feeding. Thirty Wistar rats fed with a control liquid diet were divided into control and synbiotics groups, which were respectively provided with water or synbiotics solution (1.5 g/kg body weight/day) for 2 weeks. From the 3rd to 8th week, the control group was divided into a C group (control liquid diet + water) and an E group (ethanol liquid diet + water). The synbiotics group was separated in to three groups, SC, ASE, and PSE. The SC group was given a control liquid diet with synbiotics solution; the ASE group was given ethanol liquid diet with synbiotics solution, and the PSE group was given ethanol liquid diet and water. As the results, the E group exhibited liver damage, including increased AST and ALT activities, hepatic fatty changes, and higher CYP2E1 expression. Intestinal mRNA expressions of occludin and claudin-1 were significantly decreased and the plasma endotoxin level was significantly higher in the E group. In muscles, beclin-1 was significantly increased in the E group. Compared to the E group, the PSE and ASE groups had lower plasma ALT activities, hepatic fatty changes, and CYP2E1 expression. The PSE and ASE groups had significantly higher intestinal occludin and claudin-1 mRNA expressions and lower muscular beclin-1 expression when compared to the E group. In conclusion, synbiotics supplementation might reduce protein expression of muscle protein degradation biomarkers such as beclin-1 in rats with chronic ethanol feeding, which is speculated to be linked to the improvement of intestinal tight junction and the reduction of liver damage.
本研究旨在探讨合生素对慢性乙醇喂养大鼠肝损伤、肠道健康和肌肉减少的有益作用及其相关性。将 30 只 Wistar 大鼠用对照液体饮食喂养，分为对照组和合生剂组，分别给予水或合生剂溶液（1.5 g/kg 体重/天）2 周。从第 3 周到第 8 周，对照组分为 C 组（对照液体饮食+水）和 E 组（乙醇液体饮食+水）。合生剂组分为 SC、ASE 和 PSE 三组。SC 组给予合生素溶液对照液体饮食；ASE 组给予乙醇液体饮食合生素溶液，PSE 组给予乙醇液体饮食和水。结果显示，E 组出现肝损伤，包括 AST 和 ALT 活性升高、肝脂肪变性和 CYP2E1 表达增加。肠 occludin 和 claudin-1 的 mRNA 表达显著降低，血浆内毒素水平显著升高。在肌肉中，E 组的 beclin-1 显著增加。与 E 组相比，PSE 和 ASE 组的血浆 ALT 活性、肝脂肪变性和 CYP2E1 表达降低。与 E 组相比，PSE 和 ASE 组的肠 occludin 和 claudin-1 mRNA 表达升高，肌肉 beclin-1 表达降低。综上所述，合生素补充可能会减少慢性乙醇喂养大鼠肌肉蛋白降解标志物 beclin-1 的蛋白表达，这可能与改善肠道紧密连接和减轻肝损伤有关。
