Capozzi Loredana, Simone Domenico, Bianco Angelica, Del Sambro Laura, Rondinone Valeria, Pace Lorenzo, Manzulli Viviana, Iacobellis Michela, Parisi Antonio
Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Via Manfredonia 20, 71121 Foggia, Italy.
Cytopathology Department of the Hospital Di Venere, Via Ospedale di Venere 1, 70131 Bari, Italy.
Life (Basel). 2021 Nov 18;11(11):1259. doi: 10.3390/life11111259.
SARS-CoV-2 isolates from long-term COVID-19 patients play a significant role in understanding the mechanisms of infection and virus persistence. This study describes a SARS-CoV-2 isolate from a 53-year-old woman from Apulia (Italy), who was COVID-19 positive for approximately four months. In this paper we aimed to investigate any potential correlation between genetic mutations and clinical features of this case of infection. The viral isolate was assigned to lineage B.1.177.51 through whole-genome sequencing (WGS) and harbored a novel set of mutations on the Spike protein (V143D, del144/145 and E484K); furthermore, seroneutralization assays showed impaired response of the surveyed strain to BNT162b2 (Comirnaty) Pfizer/BioNTech vaccine-induced (average reduction of 70%) and convalescent sera (average reduction of 19.04%), when compared to VOC P.1. This study highlights the importance of genomic surveillance for the management of the COVID-19 pandemic, the relevance of monitoring of emerging SARS-CoV-2 mutations in all lineages, and the necessity of testing the response of emerging variants to available therapies and vaccines.
来自长期新冠患者的严重急性呼吸综合征冠状病毒2（SARS-CoV-2）毒株在理解感染机制和病毒持续存在方面发挥着重要作用。本研究描述了一株来自意大利普利亚大区一名53岁女性的SARS-CoV-2毒株，该女性新冠病毒检测呈阳性约四个月。在本文中，我们旨在研究该感染病例的基因突变与临床特征之间的任何潜在关联。通过全基因组测序（WGS）将该病毒毒株归类为B.1.177.51谱系，并在刺突蛋白上发现了一组新的突变（V143D、del144/145和E484K）；此外，血清中和试验表明，与变异株P.1相比，该被测毒株对辉瑞/生物科技公司的BNT162b2（Comirnaty）疫苗诱导的血清（平均降低70%）和康复期血清（平均降低19.04%）的反应受损。本研究强调了基因组监测对新冠疫情管理的重要性、监测所有谱系中新兴SARS-CoV-2突变的相关性，以及测试新兴变异株对现有疗法和疫苗反应的必要性。
