Naumenko Ekaterina, Guryanov Ivan, Zakirova Elena, Fakhrullin Rawil
Institute of Fundamental Medicine and Biology, Kazan Federal University, Kreml Uramı 18, Kazan 420008, Republic of Tatarstan, Russian Federation.
Polymers (Basel). 2021 Nov 15;13(22):3949. doi: 10.3390/polym13223949.
Here we report the use of forskolin-modified halloysite nanotubes (HNTs) as a dopant for biopolymer porous hydrogel scaffolds to impart osteoinductive properties. Forskolin is a labdane diterpenoid isolated from the Indian Coleus plant. This small molecule is widely used as a supplement in molecular biology for cell differentiation. It has been reported in some earlier publications that forskolin can activate osteodifferentiation process by cyclic adenosine monophosphate (c-AMP) signalling activation in stem cells. In presented study it was demonstrated that forskolin release from halloysite-doped scaffolds induced the osteodifferentiation of equine mesenchymal stem cells (MSCs) in vitro without addition of any specific growth factors. The reinforcement of mechanical properties of cells and intercellular space during the osteodifferentiation was demonstrated using atomic force microscopy (AFM). These clay-doped scaffolds may find applications to accelerate the regeneration of horse bone defects by inducing the processes of osteodifferentiation of endogenous MSCs.
在此，我们报道了使用福司可林修饰的埃洛石纳米管（HNTs）作为生物聚合物多孔水凝胶支架的掺杂剂，以赋予其骨诱导特性。福司可林是一种从印度彩叶草植物中分离出的半日花烷二萜。这种小分子在分子生物学中广泛用作细胞分化的补充剂。一些早期出版物报道，福司可林可通过激活干细胞中的环磷酸腺苷（c-AMP）信号来激活骨分化过程。在本研究中，证明了从埃洛石掺杂支架中释放的福司可林在不添加任何特定生长因子的情况下，可在体外诱导马间充质干细胞（MSCs）的骨分化。使用原子力显微镜（AFM）证明了在骨分化过程中细胞和细胞间空间机械性能的增强。这些粘土掺杂支架可能通过诱导内源性MSCs的骨分化过程，加速马骨缺损的再生。
