Tiron Adrian, Stan Corneliu S, Luta Gabriel, Uritu Cristina M, Vacarean-Trandafir Irina-Cezara, Stanciu Gabriela D, Coroaba Adina, Tiron Crina E
TRANSCEND Centre, Regional Institute of Oncology, 2-4 General Henri Mathias Berthelot Street, 700483 Iasi, Romania.
Department of Natural and Synthetic Polymers, "Gheorghe Asachi" Technical University of Iasi, 71 Prof. dr. docent Dimitrie Mangeron Street, 700050 Iasi, Romania.
Pharmaceutics. 2021 Nov 22;13(11):1982. doi: 10.3390/pharmaceutics13111982.
Theranostics, a novel concept in medicine, is based on the use of an agent for simultaneous diagnosis and treatment. Nanomaterials provide promising novel approaches to theranostics. Carbon Dots have been shown to exhibit anti-tumoral properties in various cancer models. The aim of the present study is to develop gadolinium, Fe, and Mn-doped N-hydroxyphthalimide-derived Carbon Dots. The resulted doped Carbon Dots should preserve the anti-tumoral properties while gaining magnetic resonance imaging properties.
Normal and cancer cell lines have been treated with doped Carbon Dots, and the cell viability has been measured. The doped Carbon Dots that exhibited the most prominent anti-tumoral effect accompanied by the lowest toxicity have been further in vivo tested. Magnetic resonance imaging evaluates both in vitro and in vivo the possibility of using doped Carbon Dots as a contrast agent.
According to the results obtained from both the in vitro and in vivo experimental models used in our study, Mn-doped Carbon Dots (Mn-CDs-NHF) exhibit anti-tumoral properties, do not significantly impair the cell viability of normal cells, and reduce lung metastasis and the volume of mammary primary tumors while allowing magnetic resonance imaging.
Our findings prove that Mn-CDs-NHF can be used as theranostics agents in pre-clinical models.
治疗诊断学是医学中的一个新概念，基于使用一种试剂进行同步诊断和治疗。纳米材料为治疗诊断学提供了有前景的新方法。已证明碳点在各种癌症模型中表现出抗肿瘤特性。本研究的目的是开发钆、铁和锰掺杂的N-羟基邻苯二甲酰亚胺衍生碳点。所得的掺杂碳点应保留抗肿瘤特性，同时获得磁共振成像特性。
用掺杂碳点处理正常细胞系和癌细胞系，并测量细胞活力。对表现出最显著抗肿瘤作用且毒性最低的掺杂碳点进行进一步的体内测试。磁共振成像评估了在体外和体内使用掺杂碳点作为造影剂的可能性。
根据我们研究中使用的体外和体内实验模型获得的结果，锰掺杂碳点（Mn-CDs-NHF）表现出抗肿瘤特性，不会显著损害正常细胞的活力，并减少肺转移和乳腺原发性肿瘤的体积，同时可用于磁共振成像。
我们的研究结果证明，Mn-CDs-NHF可在临床前模型中用作治疗诊断试剂。
