Lee Hyunbeom, Kim Hyoung Ja, Chae Hyungi, Yoon Na Eun, Jung Byung Hwa
Molecular Recognition Research Center, Korea Institute of Science and Technology, Seoul 02792, Korea.
Department of HY-KIST Bio-Convergence, Hanyang University, Seoul 04763, Korea.
Plants (Basel). 2021 Oct 25;10(11):2287. doi: 10.3390/plants10112287.
F. Schmidt (AG), is a natural product known to have anti-obesity effects, but the mechanism underlying these effects is not well documented. We hypothesized that AG may have inhibitory effects on enzymes related to lipid accumulation. Herein, AG fractions were tested against HMG-CoA reductase (HMGR) and fatty acid synthase (FAS), two important enzymes involved in cholesterol and fatty acid synthesis, respectively. We found that dicaffeoylquinic acid (DCQA) methyl esters present in AG are largely responsible for the inhibition of HMGR and FAS. Since free DCQA is a major form present in AG, we demonstrated that a simple methylation of the AG extract could increase the overall inhibitory effects against those enzymes. Through this simple process, we were able to increase the inhibitory effect by 150%. We believe that our processed AG effectively modulates the HMGR and FAS activities, providing promising therapeutic potential for cholesterol- and lipid-lowering effects.
F.施密特（研究小组）发现，AG是一种已知具有抗肥胖作用的天然产物，但其作用机制尚无充分文献记载。我们推测AG可能对与脂质积累相关的酶具有抑制作用。在此，我们针对AG组分分别测试了3-羟基-3-甲基戊二酰辅酶A还原酶（HMGR）和脂肪酸合酶（FAS），这两种酶分别参与胆固醇和脂肪酸的合成。我们发现，AG中存在的二咖啡酰奎宁酸（DCQA）甲酯是抑制HMGR和FAS的主要成分。由于游离DCQA是AG中的主要形式，我们证明对AG提取物进行简单甲基化可以增强对这些酶的整体抑制作用。通过这个简单的过程，我们能够将抑制作用提高150%。我们相信，经过处理的AG能够有效调节HMGR和FAS的活性，为降低胆固醇和血脂提供了有前景的治疗潜力。
