Vuletin Selak Gabriela, Baruca Arbeiter Alenka, Cuevas Julián, Perica Slavko, Pujic Petar, Raboteg Božiković Marina, Bandelj Dunja
Department of Plant Sciences, Institute for Adriatic Crops and Karst Reclamation, 21000 Split, Croatia.
Centre of Excellence for Biodiversity and Molecular Plant Breeding (CoE CroP-BioDiv), Svetošimunska Cesta 25, 10000 Zagreb, Croatia.
Plants (Basel). 2021 Oct 31;10(11):2356. doi: 10.3390/plants10112356.
The olive tree ( L.) is a wind-pollinated crop that exhibits an extreme alternate bearing habit. To improve fruit set, several methods have been used to determine the most successful compatible combinations of cultivars. In this study, priority is given to seed paternity analysis based on simple sequence repeats (SSRs), microsatellite markers used for the identification of potential pollen donors of cultivar 'Oblica' in a mixed olive orchard during two consecutive years. Seven microsatellite primers were successfully used to examine the paternity of olive embryos from 'Oblica' mother trees. Embryos were considered as a product of self-fertilization if only maternal alleles were present, but not a single case of self-fertilization was found among all the embryos analyzed. Two dominant pollen donors were not the closest nor the cultivars with the highest number of trees in the orchard, suggesting that cross-compatibility may have a key role in determining pollen donor success. In our earlier studies, pollen tube growth and fertilization success correlated with fruit set when controlled crosses between cultivars were performed; however, some discrepancy might appear compared to paternity analyses when mother trees have a free choice among different pollen sources from cultivars growing in their surroundings.
油橄榄（L.）是一种风媒授粉作物，具有极端的隔年结果习性。为提高坐果率，人们采用了多种方法来确定最成功的品种间亲和组合。在本研究中，重点是基于简单序列重复（SSR）进行种子父本分析，SSR是一种微卫星标记，连续两年用于在混合油橄榄果园中鉴定‘奥布利卡’品种潜在的花粉供体。七个微卫星引物成功用于检测来自‘奥布利卡’母树的油橄榄胚胎的父本。如果仅存在母本等位基因，则胚胎被视为自花受精产物，但在所有分析的胚胎中未发现一例自花受精情况。两个主要的花粉供体既不是距离最近的，也不是果园中树木数量最多的品种，这表明杂交亲和性可能在决定花粉供体的成功方面起关键作用。在我们早期的研究中，当进行品种间的控制杂交时，花粉管生长和受精成功率与坐果率相关；然而，当母树可以在周围生长的不同品种的花粉来源中自由选择时，与父本分析相比可能会出现一些差异。
