Montemurro Cinzia, Dambruoso Giovanni, Bottalico Giovanna, Sabetta Wilma
Department of Soil, Plants and Food Sciences, Faculty of Agricultural Science, University of Bari "Aldo Moro,", Bari, Italy.
SINAGRI S.r.l. - Spin off University of Bari, Bari, Italy.
Front Plant Sci. 2019 Apr 12;10:451. doi: 10.3389/fpls.2019.00451. eCollection 2019.
The morphology of olive flowers allows either self- or cross-pollination that could partially explain the existence of both reproductive features in this species. However, a high degree of self-incompatibility is reported for many olive genotypes, that could be an important reproductive barrier influencing olive yield. Due to the strong environmental influence, results of compatibility tests are often contradictory, making cultivar classification quite imprecise. In this study, the self-incompatibility value has been determined for four olive genotypes (Bella di Spagna, Coratina, Leccino, and Ogliarola barese) widespread in the Mediterranean basin. Moreover, the incompatibility relationships of cultivar Coratina with some suitable pollinizers (Leccino, Oliastro, and Picholine) have been studied in controlled crosses: the germination potential of progenies has been evaluated and the selection of cross-derived embryos has been indirectly performed by the molecular characterization of the corresponding endosperm. The results increase knowledge on factors affecting self-compatibility in olive. Moreover, they provide useful information to farmers about the most effective cultivars for the set-up of new olive grove or for graft planning. Finally, they provide a new strategy and procedure based on endosperm analysis by SSRs for an accurate, fast, and relatively cheap screening of embryos/seedlings.
油橄榄花的形态使得其既能进行自花授粉，也能进行异花授粉，这在一定程度上可以解释该物种中这两种繁殖特性的存在。然而，据报道许多油橄榄基因型具有高度的自交不亲和性，这可能是影响油橄榄产量的一个重要繁殖障碍。由于环境影响强烈，亲和性测试的结果往往相互矛盾，使得品种分类相当不准确。在本研究中，已确定了在地中海盆地广泛种植的四种油橄榄基因型（贝拉迪西班牙、科拉蒂纳、莱基诺和奥利亚罗拉巴雷塞）的自交不亲和值。此外，还在控制杂交中研究了科拉蒂纳品种与一些合适授粉品种（莱基诺、奥利阿斯托和皮肖林）的不亲和关系：评估了后代的发芽潜力，并通过相应胚乳的分子特征间接进行了杂交衍生胚的选择。这些结果增加了对影响油橄榄自交亲和性因素的了解。此外，它们为农民提供了有关建立新油橄榄园或进行嫁接规划时最有效品种的有用信息。最后，它们提供了一种基于SSR对胚乳进行分析的新策略和程序，用于准确、快速且相对廉价地筛选胚/幼苗。
