Oh Seungju, Hur Hyeyeon, Kim Yoonjae, Shin Seongcheol, Woo Hyunjeong, Choi Jonghoon, Lee Hyun Ho
Department of Chemical Engineering, Myongji University, Yongin-Si 17058, Korea.
Department of Biomedical Engineering, School of Integrative Engineering, Chung-Ang University, Seoul 06974, Korea.
Nanomaterials (Basel). 2021 Oct 28;11(11):2887. doi: 10.3390/nano11112887.
Recently, various waste microplastics sensors have been introduced in response to environmental and biological hazards posed by waste microplastics. In particular, the detrimental effects of nano-sized plastics or nanoplastics have been reported to be severe. Moreover, there have been many difficulties for sensing microplastics due to the limited methodologies for selectively recognizing nanoplastics. In this study, a customized gold nanoparticles (Au NPs) based localized surface plasmon resonance (LSPR) system having bio-mimicked peptide probes toward the nanoplastics was demonstrated. The specific determination through the oligo-peptide recognition was accomplished by chemical conjugation both on the LSPR chip's 40~50 nm Au NPs and sandwiched 5 nm Au NPs, respectively. The peptide probe could selectively bind to polystyrene (PS) nanoplastics in the forms of fragmented debris by cryo-grinding. A simple UV-Vis spectrophotometer was used to identify the LSPR sensing by primarily measuring the absorbance change and shift of absorption peak. The sandwich-binding could increase the LSPR detection sensitivity up to 60% due to consecutive plasmonic effects. In addition, microwave-boiled DI water inside of a styrofoam container was tested for putative PS nanoplastics resource as a real accessible sample. The LSPR system could be a novel protocol overcoming the limitations from conventional nanoplastic detection.
最近，针对废弃微塑料对环境和生物造成的危害，各种废弃微塑料传感器应运而生。特别是，据报道纳米级塑料或纳米塑料的有害影响十分严重。此外，由于选择性识别纳米塑料的方法有限，微塑料传感面临诸多困难。在本研究中，展示了一种定制的基于金纳米颗粒（Au NPs）的局域表面等离子体共振（LSPR）系统，该系统具有针对纳米塑料的仿生肽探针。通过寡肽识别进行的特异性测定分别通过化学偶联在LSPR芯片的40~50 nm金纳米颗粒和夹在中间的5 nm金纳米颗粒上完成。肽探针可以通过冷冻研磨以碎片形式选择性地结合到聚苯乙烯（PS）纳米塑料上。使用一台简单的紫外可见分光光度计，通过主要测量吸光度变化和吸收峰的移动来识别LSPR传感。由于连续的等离子体效应，夹心结合可将LSPR检测灵敏度提高多达60%。此外，对聚苯乙烯泡沫塑料容器内用微波煮沸的去离子水进行了测试，将其作为一种实际可获取的样品，以寻找可能的PS纳米塑料来源。LSPR系统可能是一种克服传统纳米塑料检测局限性的新方法。
