Woo Sunyoung, Kim Soojin, Kim Hyunhong, Cheon Young Woo, Yoon Seokjoo, Oh Jung-Hwa, Park Jongnam
School of Energy and Chemical Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan 44919, Korea.
Department of Predictive Toxicology, Korea Institute of Toxicology (KIT), Daejeon 34114, Korea.
Nanomaterials (Basel). 2021 Nov 14;11(11):3068. doi: 10.3390/nano11113068.
The surface charge of iron oxide nanoparticles (IONPs) plays a critical role in the interactions between nanoparticles and biological components, which significantly affects their toxicity in vitro and in vivo. In this study, we synthesized three differently charged IONPs (negative, neutral, and positive) based on catechol-derived dopamine, polyethylene glycol, carboxylic acid, and amine groups, via reversible addition-fragmentation chain transfer-mediated polymerization (RAFT polymerization) and ligand exchange. The zeta potentials of the negative, neutral, and positive IONPs were -39, -0.6, and +32 mV, respectively, and all three IONPs showed long-term colloidal stability for three months in an aqueous solution without agglomeration. The cytotoxicity of the IONPs was studied by analyzing cell viability and morphological alteration in three human cell lines, A549, Huh-7, and SH-SY5Y. Neither IONP caused significant cellular damage in any of the three cell lines. Furthermore, the IONPs showed no acute toxicity in BALB/c mice, in hematological and histological analyses. These results indicate that our charged IONPs, having high colloidal stability and biocompatibility, are viable for bio-applications.
氧化铁纳米颗粒（IONPs）的表面电荷在纳米颗粒与生物成分之间的相互作用中起着关键作用，这会显著影响它们在体外和体内的毒性。在本研究中，我们基于儿茶酚衍生的多巴胺、聚乙二醇、羧酸和胺基，通过可逆加成-断裂链转移介导的聚合反应（RAFT聚合）和配体交换，合成了三种带不同电荷的IONPs（负电荷、中性电荷和正电荷）。负电荷、中性电荷和正电荷IONPs的zeta电位分别为-39、-0.6和+32 mV，并且所有三种IONPs在水溶液中均表现出长达三个月的长期胶体稳定性，无团聚现象。通过分析三种人类细胞系A549、Huh-7和SH-SY5Y中的细胞活力和形态变化，研究了IONPs的细胞毒性。在这三种细胞系中，任何一种IONP均未造成显著的细胞损伤。此外，在血液学和组织学分析中，IONPs在BALB/c小鼠中未显示出急性毒性。这些结果表明，我们制备的带电荷IONPs具有高胶体稳定性和生物相容性，适用于生物应用。
