CA-HE50 对脂多糖/半乳糖胺诱导的 C57BL/6 小鼠急性肝损伤的保护作用比较研究。
A Comparative Study of the Hepatoprotective Effect of Extract (CA-HE50) on Lipopolysaccharide/d-galactosamine-Induced Acute Liver Injury in C57BL/6 Mice.
机构信息
Department of Biomechatronic Engineering, Sungkyunkwan University, Suwon 16419, Gyeonggi, Korea.
Department of Oriental Medicine Biotechnology, College of Life Sciences, Kyung Hee University, Yongin 17104, Gyeonggi, Korea.
出版信息
Nutrients. 2021 Nov 15;13(11):4090. doi: 10.3390/nu13114090.
Acute liver failure (ALF) refers to the sudden loss of liver function and is accompanied by several complications. In a previous study, we revealed the protective effect of 50% ethanol extract (CA-HE50) on acetaminophen-induced liver injury. In the present study, we investigate the hepatoprotective effect of CA-HE50 in a lipopolysaccharide/galactosamine (LPS-D-Gal)-induced ALF animal model and compare it to existing therapeutic silymarin, mycelia (LEM) extracts, ursodeoxycholic acid (UDCA) and dimethyl diphenyl bicarboxylate (DDB). Serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels were decreased in the CA-HE50, silymarin, LEM, UDCA and DDB groups compared to the vehicle control group. In particular, AST and ALT levels of the 200 mg/kg CA-HE50 group were significantly decreased compared to positive control groups. Lactate dehydrogenase (LDH) levels were significantly decreased in the CA-HE50, silymarin, LEM, UDCA and DDB groups compared to the vehicle control group and LDH levels of the 200 mg/kg CA-HE50 group were similar to those of the positive control groups. Superoxide dismutase (SOD) activity was significantly increased in the 100 mg/kg CA-HE50, LEM and UDCA groups compared to the vehicle control group and, in particular, the 100 mg/kg CA-HE50 group increased significantly compared to positive control groups. In addition, the histopathological lesion score was significantly decreased in the CA-HE50 and positive control groups compared with the vehicle control group and the histopathological lesion score of the 200 mg/kg CA-HE50 group was similar to that of the positive control groups. These results show that CA-HE50 has antioxidant and hepatoprotective effects at a level similar to that of silymarin, LEM, UDCA and DDB, which are known to have hepatoprotective effects; further, CA-HE50 has potential as a prophylactic and therapeutic agent in ALF.
急性肝衰竭（ALF）是指肝功能突然丧失，并伴有多种并发症。在之前的研究中，我们揭示了 50%乙醇提取物（CA-HE50）对乙酰氨基酚诱导的肝损伤的保护作用。在本研究中，我们在脂多糖/半乳糖胺（LPS-D-Gal）诱导的 ALF 动物模型中研究了 CA-HE50 的肝保护作用，并将其与现有的治疗药物水飞蓟素、灵芝菌丝体（LEM）提取物、熊去氧胆酸（UDCA）和二甲基二苯基丁二酸酯（DDB）进行了比较。与 vehicle 对照组相比，CA-HE50、水飞蓟素、LEM、UDCA 和 DDB 组的血清天冬氨酸转氨酶（AST）和丙氨酸转氨酶（ALT）水平降低。特别是，200mg/kg CA-HE50 组的 AST 和 ALT 水平明显低于阳性对照组。与 vehicle 对照组相比，CA-HE50、水飞蓟素、LEM、UDCA 和 DDB 组的乳酸脱氢酶（LDH）水平显著降低，200mg/kg CA-HE50 组的 LDH 水平与阳性对照组相似。与 vehicle 对照组相比，CA-HE50、LEM 和 UDCA 组的超氧化物歧化酶（SOD）活性显著增加，特别是 100mg/kg CA-HE50 组与阳性对照组相比显著增加。此外，与 vehicle 对照组相比，CA-HE50 和阳性对照组的组织病理学损伤评分显著降低，200mg/kg CA-HE50 组的组织病理学损伤评分与阳性对照组相似。这些结果表明，CA-HE50 具有抗氧化和肝保护作用，其水平与已知具有肝保护作用的水飞蓟素、LEM、UDCA 和 DDB 相当；此外，CA-HE50 具有作为 ALF 预防和治疗剂的潜力。
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