Institute of Applied Chemistry and Department of Chemistry, Hallym University, Chuncheon 200702, South Korea.
Biometrix Technology, Inc., 2-2 Bio Venture Plaza 56, Chuncheon 24232, South Korea.
Bioorg Chem. 2022 Feb;119:105490. doi: 10.1016/j.bioorg.2021.105490. Epub 2021 Nov 20.
The reported methods mainly use biomolecules such as antibodies, enzymes, and aptamers for biomarker detection. However, applying an abiotic fluorescent probe to detect cancer biomarkers such as carcinoembryonic antigen (CEA) has not been reported. In this regard, we conceived an abiotic fluorescent probe BIQ-1 for the rapid yet straightforward detection of CEA. The bioinformatics tools and molecular docking techniques were used to develop the probe BIQ-1 for the selective detection and quantification of CEA in a buffer matrix resembling serum. The probe BIQ-1 exhibited a limit of detection of 0.2 ng/mL for CEA in a simple cuvette-based experiment. The BIQ-1 did no show interference from the possible interfering components such as hemoglobin, intralipid, and human serum albumin (HSA) in concentrations several-fold higher (µg/mL) than CEA.
所报道的方法主要使用生物分子，如抗体、酶和适体，来检测生物标志物。然而，应用非生物荧光探针来检测癌生物标志物，如癌胚抗原（CEA），尚未见报道。在这方面，我们构思了一种非生物荧光探针 BIQ-1，用于快速而直接地检测 CEA。生物信息学工具和分子对接技术被用于开发探针 BIQ-1，以在类似于血清的缓冲基质中选择性检测和定量 CEA。探针 BIQ-1 在简单的比色皿实验中对 CEA 的检测限为 0.2ng/mL。BIQ-1 没有显示出来自可能的干扰成分，如血红蛋白、脂肪乳和人血清白蛋白（HSA）的干扰，其浓度比 CEA高几个数量级（µg/mL）。
