Department of Analytical Chemistry, Faculty of Pharmacy, Ankara University, 06560, Ankara, Turkey.
Biomaterials, Energy, Photocatalysis, Enzyme Technology, Nano & Advanced Materials, Additive Manufacturing, Environmental Applications, and Sustainability Research & Development Group (BIOENAMS R&D Group), Sakarya University, 54187, Sakarya, Turkey.
Mikrochim Acta. 2021 Nov 27;188(12):434. doi: 10.1007/s00604-021-05107-2.
A novel and sensitive voltammetric nanosensor was developed for the first time for trace level monitoring of favipiravir based on gold/silver core-shell nanoparticles (Au@Ag CSNPs) with conductive polymer poly (3,4-ethylene dioxythiophene) polystyrene sulfonate (PEDOT:PSS) and functionalized multi carbon nanotubes (F-MWCNTs) on a glassy carbon electrode (GCE). The formation of Au@Ag CSNPs/PEDOT:PSS/F-MWCNT composite was confirmed by various analytical techniques, including X-ray diffraction (XRD), ultraviolet-visible spectroscopy (UV-Vis), transmission electron microscopy (TEM), energy-dispersive X-ray spectroscopy (EDX), and field-emission scanning electron microscopy (SEM). Under the optimized conditions and at a typical working potential of + 1.23 V (vs. Ag/AgCl), the Au@Ag CSNPs/PEDOT:PSS/F-MWCNT/GCE revealed linear quantitative ranges from 0.005 to 0.009 and 0.009 to 1.95 µM with a limit of detection 0.46 nM (S/N = 3) with acceptable relative standard deviations (1.1-4.9 %) for pharmaceutical formulations, urine, and human plasma samples without applying any sample pretreatment (1.12-4.93%). The interference effect of antiviral drugs, biological compounds, and amino acids was negligible, and the sensing system demonstrated outstanding reproducibility, repeatability, stability, and reusability. The findings revealed that this assay strategy has promising applications in diagnosing FAV in clinical samples, which could be attributed to the large surface area on active sites and high conductivity of bimetallic nanocomposite.
首次基于金/银核壳纳米粒子（Au@Ag CSNPs），结合导电聚合物聚（3,4-亚乙基二氧噻吩）聚苯乙烯磺酸盐（PEDOT:PSS）和功能化多壁碳纳米管（F-MWCNTs），在玻碳电极（GCE）上开发了一种用于痕量 favipiravir 监测的新型灵敏伏安纳米传感器。通过各种分析技术，包括 X 射线衍射（XRD）、紫外-可见光谱（UV-Vis）、透射电子显微镜（TEM）、能量色散 X 射线光谱（EDX）和场发射扫描电子显微镜（SEM），证实了 Au@Ag CSNPs/PEDOT:PSS/F-MWCNT 复合材料的形成。在优化条件下，在典型工作电位+1.23 V（相对于 Ag/AgCl）下，Au@Ag CSNPs/PEDOT:PSS/F-MWCNT/GCE 显示出从 0.005 到 0.009 和从 0.009 到 1.95 µM 的线性定量范围，检测限为 0.46 nM（S/N = 3），对于药物制剂、尿液和人类血浆样品，接受度相对标准偏差（1.1-4.9%），无需进行任何样品预处理（1.12-4.93%）。抗病毒药物、生物化合物和氨基酸的干扰影响可以忽略不计，传感系统表现出出色的重现性、可重复性、稳定性和可重复性。研究结果表明，该测定策略在临床样品中诊断 FAV 具有广阔的应用前景，这归因于活性位点的大表面积和双金属纳米复合材料的高导电性。
