Li Zhaolong, Lin Zhongning, Lu Zheng, Ying Zhaoyang
Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Pudang, Jin-an District, Fuzhou, 350013, Fujian Province, China.
Agricultural Ecology Institute, Fujian Academy of Agricultural Sciences, Pudang, Jin-an District, Fuzhou, 350013, Fujian Province, China.
AMB Express. 2021 Nov 27;11(1):159. doi: 10.1186/s13568-021-01318-1.
A traditional Chinese medicine formula based on the Coix seed and Lotus seed has been used as a general treatment of malnutrition, excessive fatigue, dysfunction of the spleen and stomach, and disorders of water transport in humans in China. However, there is limited information on its effects on the gut microbiota of piglets in vivo. In this study, the mix of Coix seed and Lotus were added the diet of forty weaned piglets (local piglets), and then evaluated it's affected on the gut microbiota of piglets and on the relations within the gut bacterial community. The results indicated that this traditional Chinese medicine formula (LM) and the extract of the traditional Chinese medicine formula (LMT) downregulated pH of succus gastricus and raised pH of the ileum, and LMT obviously decreased the feed conversion ratio. Further study showed LMT and LM also significantly increased the thick and long of gastrointestinal villi. And then, 16S ribosomal DNA sequencing revealed that groups LMT and LM have higher relative abundance of the genus Lactobacillus in the colon, succus gastricus, and jejunum, which are beneficial bacteria sold as dietary supplements to aid digestion or to augment health. Meanwhile, the relative abundance levels of Prevotellaceae, Alloprevotella, and Prevotella in the colon and Clostridium in succus gastricus and jejunum were lower. These experiments highlight the usefulness of the traditional Chinese medicine formula based on the Coix seed and Lotus seed for decreasing pH in succus gastricus, for improving the structure of intestinal villi and gut microflora, and then for achieving improvements in pig production performance.
在中国，一种以薏苡仁和莲子为基础的中药配方一直被用于治疗人类的营养不良、过度疲劳、脾胃功能失调以及水液代谢紊乱。然而，关于其对仔猪肠道微生物群体内影响的信息有限。在本研究中，将薏苡仁和莲子的混合物添加到40头断奶仔猪（本地仔猪）的日粮中，然后评估其对仔猪肠道微生物群以及肠道细菌群落内部关系的影响。结果表明，这种中药配方（LM）和中药配方提取物（LMT）下调了胃液pH值，提高了回肠pH值，并且LMT明显降低了饲料转化率。进一步研究表明，LMT和LM还显著增加了胃肠道绒毛的厚度和长度。然后，16S核糖体DNA测序显示，LMT组和LM组在结肠、胃液和空肠中乳酸杆菌属的相对丰度较高，乳酸杆菌属是作为膳食补充剂出售的有益细菌，有助于消化或增强健康。同时，结肠中普雷沃氏菌科、别普雷沃氏菌属和普雷沃氏菌属以及胃液和空肠中梭菌属的相对丰度水平较低。这些实验突出了以薏苡仁和莲子为基础的中药配方在降低胃液pH值、改善肠绒毛结构和肠道微生物群方面的作用，进而提高了生猪生产性能。
