Ablashi D V, Sturzenegger S, Hunter E A, Palestine A G, Fujikawa L S, Kim M K, Nussenblatt R B, Markham P D, Salahuddin S Z
Laboratory of Cellular and Molecular Biology, National Cancer Institute, Bethesda, Maryland 20892.
J Exp Pathol. 1987 Summer;3(4):693-703.
Tears, conjunctival epithelium, and corneoscleral tissue from AIDS patients were used for the isolation of HTLV-III and also for identifying cell types which support its replication. HTLV-III was isolated from tears of AIDS patients (66.6%) by cultivation of cells and fluid from patients' eyes with fresh human mononuclear cells. The cells from the conjunctival scrapings of these patients (33.3%) revealed HTLV-III antigens by indirect immunofluorescence (IF) using anti-P24 and P15 monoclonal antibodies. HTLV-III from the cell-free supernatant of the infected mononuclear cells from two patients' cocultures were further transmitted into fresh cells. The cells from right and left central cornea, as well as limbal cornea from an asymptomatic HTLV-III antibody-positive individual and one AIDS patient revealed HTLV-III upon cocultivation. HTLV-III P15 and P24 antigens were detected in cultured primary cornea epithelial cells. The tears and conjunctival cells from a control group were found to be free of HTLV-III. Although no documented cases of AIDS have been reported in corneal transplant recipients, serologic screening of donors prior to the use of the tissues for transplantation is advisable. Our data also raises important questions regarding possible transmission of virus during ophthalmologic examination by way of examiner's hands, through instruments and during contact lens fittings. Moreover, these findings indicate the need for testing various eye disinfectants for virus inactivation and/or inhibition.
来自艾滋病患者的眼泪、结膜上皮和角膜巩膜组织被用于分离人类嗜T淋巴细胞病毒III型（HTLV - III），也用于识别支持其复制的细胞类型。通过将患者眼部的细胞和液体与新鲜人单核细胞共同培养，从艾滋病患者的眼泪中分离出了HTLV - III（66.6%）。这些患者结膜刮片的细胞（33.3%）使用抗P24和P15单克隆抗体通过间接免疫荧光法（IF）显示出HTLV - III抗原。来自两名患者共培养的感染单核细胞无细胞上清液中的HTLV - III进一步传播到新鲜细胞中。来自一名无症状HTLV - III抗体阳性个体和一名艾滋病患者的左右中央角膜以及角膜缘角膜的细胞在共培养时显示出HTLV - III。在培养的原代角膜上皮细胞中检测到了HTLV - III的P15和P24抗原。发现对照组的眼泪和结膜细胞中没有HTLV - III。尽管在角膜移植受者中尚未有艾滋病确诊病例的报道，但在将组织用于移植之前对供体进行血清学筛查是可取的。我们的数据还提出了关于在眼科检查期间病毒可能通过检查者的手、器械以及在隐形眼镜佩戴过程中传播的重要问题。此外，这些发现表明需要测试各种眼部消毒剂对病毒的灭活和/或抑制作用。
