Cheng Yi, Liu Lu, Mo Simei, Gao Jianxiong, Zhang Hongjian, Zhang Heli, Zhang Chunsheng, Song Xu, Li Lixia, Geng Zhe
Department of Physical Education, Chengdu University of Information Technology, Chengdu, China.
College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.
Evid Based Complement Alternat Med. 2021 Nov 24;2021:3027708. doi: 10.1155/2021/3027708. eCollection 2021.
Cyclophosphamide is a commonly used anticancer drug, and immunosuppression is one of the most common side effects. How to recover the immunological function is important for cyclophosphamide-treated patients. In the present study, Phellodendri Cortex polysaccharides (CPP) could enhance the proliferation of mouse spleen lymphocytes in vitro. The immunoregulatory function of CPP was then investigated in cyclophosphamide-induced immunosuppressed mice. In CPP-treated groups, mice were orally treated with CPP at doses of 1, 0.5, and 0.25 g/kg bodyweight from 1 to 11 d, respectively. The cyclophosphamide was administrated in CPP and cyclophosphamide groups from 12 to 14 d. In the cyclophosphamide and normal control groups, the mice received equal volume of saline from 1 to 14 d. The results showed that CPP (1 g/kg) could significantly increase the bodyweight of mice, even during cyclophosphamide treatment. The organ coefficients of the spleen and thymus were recovered by CPP treatment. CPP upregulated the contents of cytokines (IL-2, IL-6, IFN-, and TNF-) in serum, which were downregulated by cyclophosphamide. The mRNA levels of these cytokines were also elevated by CPP treatment in the spleen. Cyclophosphamide upregulated the expressions of NF-B p65, TLR4, and MyD88, suggesting that the NF-B signaling pathway was activated by cyclophosphamide. After CPP treatment, it was recovered to normal level. These results indicated that CPP alleviated the cyclophosphamide-induced immunosuppression.
环磷酰胺是一种常用的抗癌药物，免疫抑制是其最常见的副作用之一。如何恢复环磷酰胺治疗患者的免疫功能对他们来说很重要。在本研究中，黄柏多糖（CPP）可在体外增强小鼠脾淋巴细胞的增殖。随后在环磷酰胺诱导的免疫抑制小鼠中研究了CPP的免疫调节功能。在CPP治疗组中，小鼠在第1至11天分别以1、0.5和0.25 g/kg体重的剂量口服CPP。在第12至14天，CPP组和环磷酰胺组给予环磷酰胺。在环磷酰胺组和正常对照组中，小鼠在第1至14天接受等量的生理盐水。结果表明，即使在环磷酰胺治疗期间，CPP（1 g/kg）也能显著增加小鼠体重。CPP治疗可使脾脏和胸腺的脏器系数恢复正常。CPP上调了血清中细胞因子（IL-2、IL-6、IFN-和TNF-）的含量，这些细胞因子在环磷酰胺作用下被下调。CPP治疗还使脾脏中这些细胞因子的mRNA水平升高。环磷酰胺上调了NF-κB p65、TLR4和MyD88的表达，表明环磷酰胺激活了NF-κB信号通路。CPP治疗后，其恢复到正常水平。这些结果表明，CPP减轻了环磷酰胺诱导的免疫抑制。
