Dradjat Respati S, Sananta Panji, Rosandi Rizqi Daniar, Siahaan Lasa Dhakka
Teaching Staff of Orthopaedic and Traumatology Department, Faculty of Medicine Universitas Brawijaya, RSUD Dr. Saiful Anwar, Malang, Indonesia.
Resident of Orthopaedic and Traumatology Department, Faculty of Medicine Universitas Brawijaya, RSUD Dr. Saiful Anwar, Malang, Indonesia.
Ann Med Surg (Lond). 2021 Nov 2;71:103020. doi: 10.1016/j.amsu.2021.103020. eCollection 2021 Nov.
Bone defect (3 mm in murine model) is a condition when the bone tissue cannot undergo a natural healing process caused by severe trauma, tumor, or irradiation. A bone defect is a challenge even for experienced Orthopaedic surgeons. Stromal vascular fraction (SVF) is a heterogeneous cell population derived from adipose tissue that results from minimal manipulation of the adipose tissue itself. Several studies have elucidated the effect of either SVF on bone defect healing. However, to the author's knowledge, there is no study evaluating the effect of SVF application on fracture healing, which was measured with osteocalcin biomarker. This study aims to evaluate the effect of SVF application on bone defect healing measured with osteocalcin as a biomarker of bone healing.
This was an animal study involving twelve Wistar strain . They were divided into three groups: negative group (normal rats), positive group (rats with bone defect and treated without SVF application), and SVF group (rats with bone defect and treated with SVF application). After 30 days, the rats were sacrificed, the osteocalcin biomarkers were evaluated. This biomarker was quantified using ELISA.
Osteocalcin biomarker expressions were higher in the group treated with SVF application than those without using SVF. All comparisons of the SVF group and positive control group showed significant differences (p < 0.05).
SVF application could aid the healing process in a murine model with bone defect, marked by increased osteocalcin levels.
骨缺损（在小鼠模型中为3毫米）是指由于严重创伤、肿瘤或辐射导致骨组织无法进行自然愈合的一种情况。即使对于经验丰富的骨科医生来说，骨缺损也是一项挑战。基质血管成分（SVF）是一种源自脂肪组织的异质细胞群，它是对脂肪组织本身进行最小程度操作后获得的。多项研究已经阐明了SVF对骨缺损愈合的影响。然而，据作者所知，尚无研究评估以骨钙素生物标志物来衡量的SVF应用对骨折愈合的影响。本研究旨在评估以骨钙素作为骨愈合生物标志物来衡量的SVF应用对骨缺损愈合的影响。
这是一项涉及12只Wistar品系大鼠的动物研究。它们被分为三组：阴性组（正常大鼠）、阳性组（有骨缺损且未应用SVF进行治疗的大鼠）和SVF组（有骨缺损且应用SVF进行治疗的大鼠）。30天后，处死大鼠，评估骨钙素生物标志物。使用酶联免疫吸附测定法（ELISA）对该生物标志物进行定量分析。
应用SVF治疗的组中骨钙素生物标志物的表达高于未应用SVF的组。SVF组与阳性对照组的所有比较均显示出显著差异（p < 0.05）。
应用SVF可以促进小鼠骨缺损模型的愈合过程，其标志是骨钙素水平升高。
