Fernández Lenys, Espinoza-Montero Patricio Javier, Gallegos-Lovato María José, Bustamante Paulo, Bolaños-Méndez Diego, Sampedro Juan Diego, Rodríguez Augusto, Ortega-Gallegos Andrea, Jadan Mónica
Escuela de Ciencias Químicas, Pontificia Universidad Católica del Ecuador, Quito, Ecuador.
Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA), La Plata, Argentina.
Sci Rep. 2025 Jul 23;15(1):26715. doi: 10.1038/s41598-025-11715-2.
The use of sedentary bioindicators, such as trees, in environmental contamination monitoring is receiving increased focus. This study evaluates Theobroma cacao L. as a bioindicator for cadmium (Cd) contamination by quantifying hydrogen peroxide (H₂O₂) as an oxidative stress marker in cellular suspensions exposed to CdSO₄. Chronoamperometric measurements using platinum electrodes indicated Cd accumulation in T. cacao L. and revealed a corresponding increase in H₂O₂ production up to a threshold level, beyond which cell apoptosis occurred. These findings support the potential of T. cacao L. as a bioindicator of Cd pollution. Moreover, H₂O₂ quantification via chronoamperometry demonstrated a rapid and effective method for detecting Cd-induced oxidative stress in plant systems. Future research should explore field applications, evaluate alternative plant species, and assess long-term responses under real environmental conditions to optimize this approach for large-scale biomonitoring.
在环境污染监测中，使用诸如树木等固定生物指示剂正受到越来越多的关注。本研究通过量化过氧化氢（H₂O₂）作为暴露于硫酸镉（CdSO₄）的细胞悬浮液中的氧化应激标志物，评估可可树（Theobroma cacao L.）作为镉（Cd）污染生物指示剂的情况。使用铂电极的计时电流测量表明可可树中镉的积累，并揭示了过氧化氢产生相应增加，直至达到阈值水平，超过该阈值细胞凋亡就会发生。这些发现支持了可可树作为镉污染生物指示剂的潜力。此外，通过计时电流法对过氧化氢进行定量，证明了一种检测植物系统中镉诱导氧化应激的快速有效方法。未来的研究应探索实地应用，评估其他植物物种，并评估实际环境条件下的长期反应，以优化这种大规模生物监测方法。
